Provided by: libbio-perl-run-perl_1.7.1-3_all bug


       Bio::Tools::Run::StandAloneBlast - Object for the local execution of the NCBI BLAST
       program suite (blastall, blastpgp, bl2seq).  There is experimental support for WU-Blast
       and NCBI rpsblast.


        # Local-blast "factory object" creation and blast-parameter
        # initialization:
        @params = (-database => 'swissprot', -outfile => 'blast1.out');
        $factory = Bio::Tools::Run::StandAloneBlast->new(@params);

        # Blast a sequence against a database:
        $str = Bio::SeqIO->new(-file=>'t/amino.fa', -format => 'Fasta');
        $input = $str->next_seq();
        $input2 = $str->next_seq();
        $blast_report = $factory->blastall($input);

        # Run an iterated Blast (psiblast) of a sequence against a database:
        $factory->j(3);    # 'j' is blast parameter for # of iterations
        $factory = Bio::Tools::Run::StandAloneBlast->new(@params);
        $blast_report = $factory->blastpgp($input);

        # Use blast to align 2 sequences against each other:
        $factory = Bio::Tools::Run::StandAloneBlast->new(-outfile => 'bl2seq.out');
        $factory->bl2seq($input, $input2);

        # Experimental support for WU-Blast 2.0
        my $factory = Bio::Tools::Run::StandAloneBlast->new(-program =>"wublastp",
                                                            -database =>"swissprot",
                                                            -e => 1e-20);
        my $blast_report = $factory->wublast($seq);

        # Experimental support for NCBI rpsblast
        my $factory = Bio::Tools::Run::StandAloneBlast->new(-db => 'CDD/Cog',
                                                            -expect => 0.001);
        $factory->F('T'); # turn on SEG filtering of query sequence
        my $blast_report = $factory->rpsblast($seq);

        # Use the experimental fast Blast parser, 'blast_pull'
        my $factory = Bio::Tools::Run::StandAloneBlast->new(-_READMETHOD =>'blast_pull',

        # Various additional options and input formats are available,
        # see the DESCRIPTION section for details.


       This DESCRIPTION only documents Bio::Tools::Run::StandAloneBlast, a Bioperl object for
       running the NCBI standAlone BLAST package. Blast itself is a large & complex program - for
       more information regarding BLAST, please see the BLAST documentation which accompanies the
       BLAST distribution. BLAST is available from

       A source of confusion in documenting a BLAST interface is that the term "program" is used
       in - at least - three different ways in the BLAST documentation. In this DESCRIPTION,
       "program" will refer to the BLAST routine set by the BLAST "-p" parameter that can be set
       to blastn, blastp, tblastx etc. We will use the term Blast "executable" to refer to the
       various different executable files that may be called - ie.  blastall, blastpgp or bl2seq.
       In addition, there are several BLAST capabilities, which are also referred to as
       "programs", and are implemented by using specific combinations of BLAST executables,
       programs and parameters. They will be referred by their specific names - eg PSIBLAST and

       Before running StandAloneBlast it is necessary: to install BLAST on your system, to edit
       set the environmental variable $BLASTDIR or your $PATH variable to point to the BLAST
       directory, and to ensure that users have execute privileges for the BLAST program.

       If the databases which will be searched by BLAST are located in the data subdirectory of
       the blast program directory (the default installation location), StandAloneBlast will find
       them; however, if the database files are located in any other location, environmental
       variable $BLASTDATADIR will need to be set to point to that directory.

       The use of the StandAloneBlast module is as follows: Initially, a local blast "factory
       object" is created. The constructor may be passed an optional array of (non-default)
       parameters to be used by the factory, eg:

        @params = (-program => 'blastn', -database => 'ecoli.nt');
        $factory = Bio::Tools::Run::StandAloneBlast->new(@params);

       Any parameters not explicitly set will remain as the defaults of the BLAST executable.
       Note each BLAST executable has somewhat different parameters and options. See the BLAST
       Documentation for a description or run the BLAST executable from the command line followed
       solely with a "-" to see a list of options and default values for that executable; eg
       >blastall -.

       BLAST parameters can be changed and/or examined at any time after the factory has been
       created. The program checks that any parameter/switch being set/read is valid. Except
       where specifically noted, StandAloneBlast uses the same single-letter, case-sensitive
       parameter names as the actual blast program. Currently no checks are included to verify
       that parameters are of the proper type (e.g. string or numeric) or that their values are
       within the proper range.

       As an example, to change the value of the Blast parameter 'e' ('e' is the parameter for
       expectation-value cutoff)

         $expectvalue = 0.01;

       Note that for improved script readibility one can modify the name of the (ncbi) BLAST
       parameters as desired as long as the initial letter (and case) of the parameter are
       preserved, e.g.:


       Unfortunately, some of the BLAST parameters are not the single letter one might expect (eg
       "iteration round" in blastpgp is 'j').  Again one can check by using, for example:

         > blastpgp -

       Wublast parameters need to be complete (ie. don't truncate them to their first letter),
       but are case-insensitive.

       Once the factory has been created and the appropriate parameters set, one can call one of
       the supported blast executables. The input sequence(s) to these executables may be fasta
       file(s) as described in the BLAST documentation.

         $inputfilename = 't/testquery.fa';
         $blast_report = $factory->blastall($inputfilename);

       In addition, sequence input may be in the form of either a Bio::Seq object or (a reference
       to) an array of Bio::Seq objects, e.g.:

         $input = Bio::Seq->new(-id => "test query",
                                -seq => "ACTACCCTTTAAATCAGTGGGGG");
         $blast_report = $factory->blastall($input);

       NOTE: Use of the BPlite method has been deprecated and is no longer supported.

       For blastall and non-psiblast blastpgp runs, report object is a Bio::SearchIO object,
       selected by the user with the parameter _READMETHOD. The leading underscore is needed to
       distinguish this option from options which are passed to the BLAST executable. The default
       parser is Bio::SearchIO::blast. In any case, the "raw" blast report is also available. The
       filename is set by the 'outfile' parameter and has the default value of "blastreport.out".

       For psiblast execution in the BLAST "jumpstart" mode, the program must be passed (in
       addition to the query sequence itself) an alignment containing the query sequence (in the
       form of a SimpleAlign object) as well as a "mask" specifying at what residues position-
       specific scoring matrices (PSSMs) are to used and at what residues default scoring
       matrices (eg BLOSUM) are to be used. See psiblast documentation for more details. The mask
       itself is a string of 0's and 1's which is the same length as each sequence in the
       alignment and has a "1" at locations where (PSSMs) are to be used and a "0" at all other
       locations. So for example:

         $str = Bio::AlignIO->new(-file => "cysprot.msf",
                                  -format => 'msf');
         $aln = $str->next_aln();
         $len = $aln->length_aln();
         $mask = '1' x $len;
         # simple case where PSSM's to be used at all residues
         $report = $factory->blastpgp("cysprot1.fa", $aln, $mask);

       For bl2seq execution, can be combined with to directly
       produce a SimpleAlign object from the alignment of the two sequences produced by bl2seq as

         # Get 2 sequences
         $str = Bio::SeqIO->new(-file=>'t/amino.fa' , -format => 'Fasta');
         my $seq3 = $str->next_seq();
         my $seq4 = $str->next_seq();

         # Run bl2seq on them
         $factory = Bio::Tools::Run::StandAloneBlast->new(-program => 'blastp',
                                                          -outfile => 'bl2seq.out');
         my $bl2seq_report = $factory->bl2seq($seq3, $seq4);

         # Use to create a SimpleAlign object from the bl2seq report
         $str = Bio::AlignIO->new(-file=> 'bl2seq.out',-format => 'bl2seq');
         $aln = $str->next_aln();

       For more examples of syntax and use of, the user is encouraged to run
       the scripts in the bioperl examples/tools directory and
       StandAloneBlast.t in the bioperl t/ directory.


   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send
       your comments and suggestions preferably to one of the Bioperl mailing lists.  Your
       participation is much appreciated.
                  - General discussion  - About the mailing lists

       Please direct usage questions or support issues to the mailing list:

       rather than to the module maintainer directly. Many experienced and reponsive experts will
       be able look at the problem and quickly address it. Please include a thorough description
       of the problem with code and data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their
       resolution.  Bug reports can be submitted via the web:

AUTHOR - Peter Schattner

       Email schattner at

MAINTAINER - Torsten Seemann

       Email torsten at


       Sendu Bala (reimplementation)


       The rest of the documentation details each of the object methods. Internal methods are
       usually preceded with a _

        Title   : new
        Usage   : my $obj = Bio::Tools::Run::StandAloneBlast->new();
        Function: Builds a newBio::Tools::Run::StandAloneBlast object
        Returns : Bio::Tools::Run::StandAloneNCBIBlast or StandAloneWUBlast
        Args    : -quiet => boolean # make program execution quiet
                  -_READMETHOD => 'BLAST' (default, synonym 'SearchIO') || 'blast_pull'
                                  # the parsing method, case insensitive

       Essentially all BLAST parameters can be set via  Some of the most
       commonly used parameters are listed below. All parameters have defaults and are optional
       except for -p in those programs that have it. For a complete listing of settable
       parameters, run the relevant executable BLAST program with the option "-" as in blastall -
       Note that the input parameters (-i, -j, -input) should not be set directly by you: this
       module sets them when you call one of the executable methods.


         -p  Program Name [String]
               Input should be one of "blastp", "blastn", "blastx",
               "tblastn", or "tblastx".
         -d  Database [String] default = nr
               The database specified must first be formatted with formatdb.
               Multiple database names (bracketed by quotations) will be accepted.
               An example would be -d "nr est"
         -e  Expectation value (E) [Real] default = 10.0
         -o  BLAST report Output File [File Out]  Optional,
                   default = ./blastreport.out ; set by
         -S  Query strands to search against database (for blast[nx], and tblastx). 3 is both, 1 is top, 2 is bottom [Integer]
                   default = 3

       Blastpgp (including Psiblast)

         -j  is the maximum number of rounds (default 1; i.e., regular BLAST)
         -h  is the e-value threshold for including sequences in the
                   score matrix model (default 0.001)
         -c  is the "constant" used in the pseudocount formula specified in the paper (default 10)
         -B  Multiple alignment file for PSI-BLAST "jump start mode"  Optional
         -Q  Output File for PSI-BLAST Matrix in ASCII [File Out]  Optional


         -d  Database [String] default = (none - you must specify a database)
               The database specified must first be formatted with formatdb.
               Multiple database names (bracketed by quotations) will be accepted.
               An example would be -d "Cog Smart"
         -e  Expectation value (E) [Real] default = 10.0
         -o  BLAST report Output File [File Out]  Optional,
                   default = ./blastreport.out ; set by


         -p  Program name: blastp, blastn, blastx. For blastx 1st argument should be nucleotide [String]
           default = blastp
         -o  alignment output file [File Out] default = stdout
         -e  Expectation value (E) [Real]  default = 10.0
         -S  Query strands to search against database (blastn only).  3 is both, 1 is top, 2 is bottom [Integer]
           default = 3


         -p Program Name [String]
               Input should be one of "wublastp", "wublastn", "wublastx",
               "wutblastn", or "wutblastx".
         -d  Database [String] default = nr
               The database specified must first be formatted with xdformat.
         -E  Expectation value (E) [Real] default = 10.0
         -o  BLAST report Output File [File Out]  Optional,
                   default = ./blastreport.out ; set by

        Title   : executable
        Usage   : my $exe = $blastfactory->executable('blastall');
        Function: Finds the full path to the executable
        Returns : string representing the full path to the exe
        Args    : [optional] name of executable to set path to
                  [optional] boolean flag whether or not warn when exe is not found

        Title   : program_dir
        Usage   : my $dir = $factory->program_dir();
        Function: Abstract get method for dir of program.
        Returns : string representing program directory
        Args    : none

        Title   :  _setinput
        Usage   :  Internal function, not to be called directly
        Function:   Create input file(s) for Blast executable
        Example :
        Returns : name of file containing Blast data input
        Args    : Seq object reference or input file name

Bio::Tools::Run::WrapperBase methods

        Title   : no_param_checks
        Usage   : $obj->no_param_checks($newval)
        Function: Boolean flag as to whether or not we should
                  trust the sanity checks for parameter values
        Returns : value of no_param_checks
        Args    : newvalue (optional)

        Title   : save_tempfiles
        Usage   : $obj->save_tempfiles($newval)
        Returns : value of save_tempfiles
        Args    : newvalue (optional)

        Title   : outfile_name
        Usage   : my $outfile = $tcoffee->outfile_name();
        Function: Get/Set the name of the output file for this run
                  (if you wanted to do something special)
        Returns : string
        Args    : [optional] string to set value to

        Title   : tempdir
        Usage   : my $tmpdir = $self->tempdir();
        Function: Retrieve a temporary directory name (which is created)
        Returns : string which is the name of the temporary directory
        Args    : none

        Title   : cleanup
        Usage   : $tcoffee->cleanup();
        Function: Will cleanup the tempdir directory after a PAML run
        Returns : none
        Args    : none

        Title   : io
        Usage   : $obj->io($newval)
        Function:  Gets a Bio::Root::IO object
        Returns : Bio::Root::IO
        Args    : none