Provided by: libbio-perl-perl_1.7.2-2_all bug

NAME

       Bio::Search::HSP::GenericHSP - A "Generic" implementation of a High Scoring Pair

SYNOPSIS

           my $hsp = Bio::Search::HSP::GenericHSP->new( -algorithm => 'blastp',
                                                       -evalue    => '1e-30',
                                                       );

           $r_type = $hsp->algorithm;

           $pvalue = $hsp->p();

           $evalue = $hsp->evalue();

           $frac_id = $hsp->frac_identical( ['query'|'hit'|'total'] );

           $frac_cons = $hsp->frac_conserved( ['query'|'hit'|'total'] );

           $gaps = $hsp->gaps( ['query'|'hit'|'total'] );

           $qseq = $hsp->query_string;

           $hseq = $hsp->hit_string;

           $homo_string = $hsp->homology_string;

           $len = $hsp->length( ['query'|'hit'|'total'] );

           $len = $hsp->length( ['query'|'hit'|'total'] );

           $rank = $hsp->rank;

       # TODO: Describe how to configure a SearchIO stream so that it generates #
       GenericHSP objects.

DESCRIPTION

       This implementation is "Generic", meaning it is is suitable for holding information about
       High Scoring pairs from most Search reports such as BLAST and FastA.  Specialized objects
       can be derived from this.

       Unless you're writing a parser, you won't ever need to create a GenericHSP or any other
       HSPI-implementing object. If you use the SearchIO system, HSPI objects are created
       automatically from a SearchIO stream which returns Bio::Search::Result::ResultI objects
       and you get the HSPI objects via the ResultI API.

       For documentation on what you can do with GenericHSP (and other HSPI objects), please see
       the API documentation in Bio::Search::HSP::HSPI.

FEEDBACK

   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send
       your comments and suggestions preferably to the Bioperl mailing list.  Your participation
       is much appreciated.

         bioperl-l@bioperl.org                  - General discussion
         http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and reponsive experts will
       be able look at the problem and quickly address it. Please include a thorough description
       of the problem with code and data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their
       resolution. Bug reports can be submitted via the web:

         https://github.com/bioperl/bioperl-live/issues

AUTHOR - Jason Stajich and Steve Chervitz

       Email jason-at-bioperl.org Email sac-at-bioperl.org

CONTRIBUTORS

       Sendu Bala, bix@sendu.me.uk

APPENDIX

       The rest of the documentation details each of the object methods.  Internal methods are
       usually preceded with a _

   new
        Title   : new
        Usage   : my $obj = Bio::Search::HSP::GenericHSP->new();
        Function: Builds a new Bio::Search::HSP::GenericHSP object
        Returns : Bio::Search::HSP::GenericHSP
        Args    : -algorithm => algorithm used (BLASTP, TBLASTX, FASTX, etc)
                  -evalue    => evalue
                  -pvalue    => pvalue
                  -bits      => bit value for HSP
                  -score     => score value for HSP (typically z-score but depends on
                                                     analysis)
                  -hsp_length=> Length of the HSP (including gaps)
                  -identical => # of residues that that matched identically
                  -percent_identity => (optional) percent identity
                  -conserved => # of residues that matched conservatively
                                  (only protein comparisons;
                                   conserved == identical in nucleotide comparisons)
                  -hsp_gaps   => # of gaps in the HSP
                  -query_gaps => # of gaps in the query in the alignment
                  -hit_gaps   => # of gaps in the subject in the alignment
                  -query_name  => HSP Query sequence name (if available)
                  -query_start => HSP Query start (in original query sequence coords)
                  -query_end   => HSP Query end (in original query sequence coords)
                  -query_length=> total length of the query sequence
                  -query_seq   => query sequence portion of the HSP
                  -query_desc  => textual description of the query
                  -hit_name    => HSP Hit sequence name (if available)
                  -hit_start   => HSP Hit start (in original hit sequence coords)
                  -hit_end     => HSP Hit end (in original hit sequence coords)
                  -hit_length  => total length of the hit sequence
                  -hit_seq     => hit sequence portion of the HSP
                  -hit_desc    => textual description of the hit
                  -homology_seq=> homology sequence for the HSP
                  -hit_frame   => hit frame (only if hit is translated protein)
                  -query_frame => query frame (only if query is translated protein)
                  -rank        => HSP rank
                  -links       => HSP links information (WU-BLAST only)
                  -hsp_group   => HSP Group informat (WU-BLAST only)
                  -gap_symbol  => symbol representing a gap (default = '-')
                  -hit_features=> string of features found in or near HSP hit
                                  region (reported in some BLAST text output,
                                  v. 2.2.13 and up)
                  -stranded    => If the algorithm isn't known (i.e. defaults to
                                  'generic'), setting this will indicate start/end
                                  coordinates are to be used to determine the strand
                                  for 'query', 'subject', 'hit', 'both', or 'none'
                                  (default = 'none')

   Bio::Search::HSP::HSPI methods
       Implementation of Bio::Search::HSP::HSPI methods follow

   algorithm
        Title   : algorithm
        Usage   : my $r_type = $hsp->algorithm
        Function: Obtain the name of the algorithm used to obtain the HSP
        Returns : string (e.g., BLASTP)
        Args    : [optional] scalar string to set value

   pvalue
        Title   : pvalue
        Usage   : my $pvalue = $hsp->pvalue();
        Function: Returns the P-value for this HSP or undef
        Returns : float or exponential (2e-10)
                  P-value is not defined with NCBI Blast2 reports.
        Args    : [optional] numeric to set value

   evalue
        Title   : evalue
        Usage   : my $evalue = $hsp->evalue();
        Function: Returns the e-value for this HSP
        Returns : float or exponential (2e-10)
        Args    : [optional] numeric to set value

   frac_identical
        Title   : frac_identical
        Usage   : my $frac_id = $hsp->frac_identical( ['query'|'hit'|'total'] );
        Function: Returns the fraction of identitical positions for this HSP
        Returns : Float in range 0.0 -> 1.0
        Args    : arg 1:  'query' = num identical / length of query seq (without gaps)
                          'hit'   = num identical / length of hit seq (without gaps)
                                    synonyms: 'sbjct', 'subject'
                          'total' = num identical / length of alignment (with gaps)
                                    synonyms: 'hsp'
                          default = 'total'
                  arg 2: [optional] frac identical value to set for the type requested
        Note    : for translated sequences, this adjusts the length accordingly

   frac_conserved
        Title    : frac_conserved
        Usage    : my $frac_cons = $hsp->frac_conserved( ['query'|'hit'|'total'] );
        Function : Returns the fraction of conserved positions for this HSP.
                   This is the fraction of symbols in the alignment with a
                   positive score.
        Returns : Float in range 0.0 -> 1.0
        Args    : arg 1: 'query' = num conserved / length of query seq (without gaps)
                         'hit'   = num conserved / length of hit seq (without gaps)
                                    synonyms: 'sbjct', 'subject'
                         'total' = num conserved / length of alignment (with gaps)
                                    synonyms: 'hsp'
                         default = 'total'
                  arg 2: [optional] frac conserved value to set for the type requested

   gaps
        Title    : gaps
        Usage    : my $gaps = $hsp->gaps( ['query'|'hit'|'total'] );
        Function : Get the number of gap characters in the query, hit, or total alignment.
        Returns  : Integer, number of gaps or 0 if none
        Args     : arg 1: 'query' = num gap characters in query seq
                          'hit'   = num gap characters in hit seq; synonyms: 'sbjct', 'subject'
                          'total' = num gap characters in whole alignment;  synonyms: 'hsp'
                          default = 'total'
                   arg 2: [optional] integer gap value to set for the type requested

   query_string
        Title   : query_string
        Usage   : my $qseq = $hsp->query_string;
        Function: Retrieves the query sequence of this HSP as a string
        Returns : string
        Args    : [optional] string to set for query sequence

   hit_string
        Title   : hit_string
        Usage   : my $hseq = $hsp->hit_string;
        Function: Retrieves the hit sequence of this HSP as a string
        Returns : string
        Args    : [optional] string to set for hit sequence

   homology_string
        Title   : homology_string
        Usage   : my $homo_string = $hsp->homology_string;
        Function: Retrieves the homology sequence for this HSP as a string.
                : The homology sequence is the string of symbols in between the
                : query and hit sequences in the alignment indicating the degree
                : of conservation (e.g., identical, similar, not similar).
        Returns : string
        Args    : [optional] string to set for homology sequence

   consensus_string
        Title   : consensus_string
        Usage   : my $cs_string = $hsp->consensus_string;
        Function: Retrieves the consensus structure line for this HSP as a string (HMMER).
                : If the model had any consensus structure or reference line annotation
                : that it inherited from a multiple alignment (#=GC SS cons,
                : #=GC RF annotation in Stockholm files), that information is shown
                : as CS or RF annotation line.
        Returns : string
        Args    : [optional] string to set for consensus structure

   posterior_string
        Title   : posterior_string
        Usage   : my $pp_string = $hsp->posterior_string;
        Function: Retrieves the posterior probability line for this HSP as a string (HMMer3).
                : The posterior probability is the string of symbols at the bottom
                : of the alignment indicating the expected accuracy of each aligned residue.
                : A 0 means 0-5%, 1 means 5-15%, and so on; 9 means 85-95%,
                : and a * means 95-100% posterior probability.
        Returns : string
        Args    : [optional] string to set for posterior probability

   length
        Title    : length
        Usage    : my $len = $hsp->length( ['query'|'hit'|'total'] );
        Function : Returns the length of the query or hit in the alignment
                   (without gaps)
                   or the aggregate length of the HSP (including gaps;
                   this may be greater than either hit or query )
        Returns  : integer
        Args     : arg 1: 'query' = length of query seq (without gaps)
                          'hit'   = length of hit seq (without gaps) (synonyms: sbjct, subject)
                          'total' = length of alignment (with gaps)
                          default = 'total'
                   arg 2: [optional] integer length value to set for specific type

   hsp_length
        Title   : hsp_length
        Usage   : my $len = $hsp->hsp_length()
        Function: shortcut  length('hsp')
        Returns : floating point between 0 and 100
        Args    : none

   percent_identity
        Title   : percent_identity
        Usage   : my $percentid = $hsp->percent_identity()
        Function: Returns the calculated percent identity for an HSP
        Returns : floating point between 0 and 100
        Args    : none

   frame
        Title   : frame
        Usage   : my ($qframe, $hframe) = $hsp->frame('list',$queryframe,$subjectframe)
        Function: Set the Frame for both query and subject and insure that
                  they agree.
                  This overrides the frame() method implementation in
                  FeaturePair.
        Returns : array of query and subject frame if return type wants an array
                  or query frame if defined or subject frame if not defined
        Args    : 'hit' or 'subject' or 'sbjct' to retrieve the frame of the subject (default)
                  'query' to retrieve the query frame
                  'list' or 'array' to retrieve both query and hit frames together
        Note    : Frames are stored in the GFF way (0-2) not 1-3
                  as they are in BLAST (negative frames are deduced by checking
                                        the strand of the query or hit)

   get_aln
        Title   : get_aln
        Usage   : my $aln = $hsp->gel_aln
        Function: Returns a L<Bio::SimpleAlign> object representing the HSP alignment
        Returns : L<Bio::SimpleAlign>
        Args    : none

   num_conserved
        Title   : num_conserved
        Usage   : $obj->num_conserved($newval)
        Function: returns the number of conserved residues in the alignment
        Returns : integer
        Args    : integer (optional)

   num_identical
        Title   : num_identical
        Usage   : $obj->num_identical($newval)
        Function: returns the number of identical residues in the alignment
        Returns : integer
        Args    : integer (optional)

   rank
        Usage     : $hsp->rank( [string] );
        Purpose   : Get the rank of the HSP within a given Blast hit.
        Example   : $rank = $hsp->rank;
        Returns   : Integer (1..n) corresponding to the order in which the HSP
                    appears in the BLAST report.

   seq_inds
        Title   : seq_inds
        Purpose   : Get a list of residue positions (indices) for all identical
                  : or conserved residues in the query or sbjct sequence.
        Example   : @s_ind = $hsp->seq_inds('query', 'identical');
                  : @h_ind = $hsp->seq_inds('hit', 'conserved');
                  : @h_ind = $hsp->seq_inds('hit', 'conserved-not-identical');
                  : @h_ind = $hsp->seq_inds('hit', 'conserved', 1);
        Returns   : List of integers
                  : May include ranges if collapse is true.
        Argument  : seq_type  = 'query' or 'hit' or 'sbjct'  (default = query)
                  :  ('sbjct' is synonymous with 'hit')
                  : class     = 'identical' - identical positions
                  :             'conserved' - conserved positions
                  :             'nomatch'   - mismatched residue or gap positions
                  :             'mismatch'  - mismatched residue positions (no gaps)
                  :             'gap'       - gap positions only
                  :             'frameshift'- frameshift positions only
                  :             'conserved-not-identical' - conserved positions w/o
                  :                            identical residues
                  :             The name can be shortened to 'id' or 'cons' unless
                  :             the name is .  The default value is
                  :             'identical'
                  :
                  : collapse  = boolean, if true, consecutive positions are merged
                  :             using a range notation, e.g., "1 2 3 4 5 7 9 10 11"
                  :             collapses to "1-5 7 9-11". This is useful for
                  :             consolidating long lists. Default = no collapse.
                  :
        Throws    : n/a.
        Comments  : For HSPs partially or completely derived from translated sequences
                  : (TBLASTN, BLASTX, TBLASTX, or similar), some positional data
                  : cannot easily be attributed to a single position (i.e. the
                  : positional data is ambiguous).  In these cases all three codon
                  : positions are reported.  Under these conditions you can check
                  : ambiguous_seq_inds() to determine whether the query, subject,
                  : or both are ambiguous.
                  :
       See Also   : L<Bio::Search::SearchUtils::collapse_nums()|Bio::Search::SearchUtils>,
                    L<Bio::Search::Hit::HitI::seq_inds()|Bio::Search::Hit::HitI>

   ambiguous_seq_inds
        Title     : ambiguous_seq_inds
        Purpose   : returns a string denoting whether sequence indices for query,
                  : subject, or both are ambiguous
        Returns   : String; 'query', 'subject', 'query/subject', or empty string ''
        Argument  : none
        Comments  : For HSPs partially or completely derived from translated sequences
                  : (TBLASTN, BLASTX, TBLASTX, or similar), some positional data
                  : cannot easily be attributed to a single position (i.e. the
                  : positional data is ambiguous).  In these cases all three codon
                  : positions are reported when using seq_inds().  Under these
                  : conditions you can check ambiguous_seq_inds() to determine whether
                  : the query, subject, or both are ambiguous.
       See Also   : L<Bio::Search::Hit::HSPI::seq_inds()>

   Inherited from Bio::SeqFeature::SimilarityPair
       These methods come from Bio::SeqFeature::SimilarityPair

   query
        Title   : query
        Usage   : my $query = $hsp->query
        Function: Returns a SeqFeature representing the query in the HSP
        Returns : L<Bio::SeqFeature::Similarity>
        Args    : [optional] new value to set

   hit
        Title   : hit
        Usage   : my $hit = $hsp->hit
        Function: Returns a SeqFeature representing the hit in the HSP
        Returns : L<Bio::SeqFeature::Similarity>
        Args    : [optional] new value to set

   significance
        Title   : significance
        Usage   : $evalue = $obj->significance();
                  $obj->significance($evalue);
        Function: Get/Set the significance value
        Returns : numeric
        Args    : [optional] new value to set

   strand
        Title   : strand
        Usage   : $hsp->strand('query')
        Function: Retrieves the strand for the HSP component requested
        Returns : +1 or -1
        Args    : 'hit' or 'subject' or 'sbjct' to retrieve the strand of the subject,
                  'query' to retrieve the query strand (default)

   score
        Title   : score
        Usage   : $score = $obj->score();
                  $obj->score($value);
        Function: Get/Set the score value
        Returns : numeric
        Args    : [optional] new value to set

   bits
        Title   : bits
        Usage   : $bits = $obj->bits();
                  $obj->bits($value);
        Function: Get/Set the bits value
        Returns : numeric
        Args    : [optional] new value to set

Private methods

   _calculate_seq_positions
        Title   : _calculate_seq_positions
        Usage   : $self->_calculate_seq_positions
        Function: Internal function
        Returns :
        Args    :

   n
       See documentation in Bio::Search::HSP::HSPI::n()

   range
       See documentation in Bio::Search::HSP::HSPI::range()

   links
        Title   : links
        Usage   : $obj->links($newval)
        Function: Get/Set the Links value (from WU-BLAST)
                  Indicates the placement of the alignment in the group of HSPs
        Returns : Value of links
        Args    : On set, new value (a scalar or undef, optional)

   hsp_group
        Title   : hsp_group
        Usage   : $obj->hsp_group($newval)
        Function: Get/Set the Group value (from WU-BLAST)
                  Indicates a grouping of HSPs
        Returns : Value of group
        Args    : On set, new value (a scalar or undef, optional)

   hit_features
        Title   : hit_features
        Usage   : $obj->hit_features($newval)
        Function: Get/Set the HSP hit feature string (from some BLAST 2.2.13 text
                  output), which is a string of overlapping or nearby features in HSP
                  hit
        Returns : Value of hit features, if present
        Args    : On set, new value (a scalar or undef, optional)

Brief introduction on cigar string

       NOTE: the concept is originally from EnsEMBL docs at
       http://may2005.archive.ensembl.org/Docs/wiki/html/EnsemblDocs/CigarFormat.html Please
       append to these docs if you have a better definition.

       Sequence alignment hits can be stored in a database as ungapped alignments.  This imposes
       2 major constraints on alignments:

       a) alignments for a single hit record require multiple rows in the database, and b) it is
       not possible to accurately retrieve the exact original alignment.

       Alternatively, sequence alignments can be stored as gapped alignments using the CIGAR line
       format (where CIGAR stands for Concise Idiosyncratic Gapped Alignment Report).

       In the cigar line format alignments are stored as follows:

       M: Match D: Deletion I: Insertion

       An example of an alignment for a hypthetical protein match is shown below:

       Query:   42 PGPAGLP----GSVGLQGPRGLRGPLP-GPLGPPL...

                   PG    P    G     GP   R      PLGP

       Sbjct: 1672 PGTP*TPLVPLGPWVPLGPSSPR--LPSGPLGPTD...

       protein_align_feature table as the following cigar line:

       7M4D12M2I2MD7M

   cigar_string
         Name:     cigar_string
         Usage:    $cigar_string = $hsp->cigar_string
         Function: Generate and return cigar string for this HSP alignment
         Args:     No input needed
         Return:   a cigar string

   generate_cigar_string
         Name:     generate_cigar_string
         Usage:    my $cigar_string = Bio::Search::HSP::GenericHSP::generate_cigar_string ($qstr, $hstr);
         Function: generate cigar string from a simple sequence of alignment.
         Args:     the string of query and subject
         Return:   cigar string