Provided by: wigeon_20101212+dfsg1-2_all bug


       wigeon - reimplementation of the Pintail 16S DNA anomaly detection utility


       WigeoN  examines  the  sequence  conservation  between  a  query  and  a trusted reference
       sequence, both in NAST alignment format. Based on the sequence identity between the  query
       and  the reference sequence, there is an expected amount of variation among the alignment.
       If the observed variation is  greater  than  the  95%  quantile  of  the  distribution  of
       variation observed between non-anomalous sequences, then it is flagged as an anomaly.

       WigeoN  is  a  flexible  command-line based reimplementation of the Pintail algorithm Appl
       Environ Microbiol. 2005 Dec;7112:7724-36.

       WigeoN is useful for flagging chimeras and anomalies only in  near  full-length  16S  rRNA
       sequences. WigeoN lacks sensitivity with sequences less than 1000 bp.

       To run WigeoN, you need NAST-formatted sequences generated by the nast-ier utility.

       WigeoN is  part of the microbiomeutil suite.


              multi-fasta file containing query sequences in alignment format

              db in NAST format

              db in fasta format (megablast formatted)

              default 1: uses only the single best match.



              cd to exec_dir before running



       This  manual  page  was written by Andreas Tille <> but can be freely used
       for any other distribution.