Provided by: emboss_6.6.0+dfsg-7ubuntu2_amd64 bug


       primers - Simple version of primer3 to pick PCR primers


       primers -sequence seqall [-targetregion range] [-minsize integer] [-maxsize integer]
               [-maxdifftm float] [-explainflag boolean] [-numreturn integer] -outfile outfile

       primers -help


       primers is a command line program from EMBOSS (“the European Molecular Biology Open
       Software Suite”). It is part of the "Nucleic:Primers" command group(s).


   Input section
       -sequence seqall
           The sequence from which to choose primers. The sequence must be presented 5' -> 3'

   Additional section
       -targetregion range
           If one or more Targets is specified then a legal primer pair must flank at least one
           of them. A Target might be a simple sequence repeat site (for example a CA repeat) or
           a single-base-pair polymorphism. The value should be a space-separated list of
           <start>,<end> pairs where <start> is the index of the first base of a Target, and
           <end> is the last E.g. 50,51 requires primers to surround the 2 bases at positions 50
           and 51.

       -minsize integer
           Minimum acceptable length of a primer. Must be greater than 0 and less than or equal
           to MAX-SIZE. Default value: 18

       -maxsize integer
           Maximum acceptable length (in bases) of a primer. Currently this parameter cannot be
           larger than 35. This limit is governed by the maximum oligo size for which Primer3's
           melting-temperature is valid. Default value: 27

       -maxdifftm float
           Maximum acceptable (unsigned) difference between the melting temperatures of the
           forward and reverse primers. Default value: 100.0

   Output section
       -explainflag boolean
           If this flag is non-0, produce LEFT-EXPLAIN and RIGHT-EXPLAIN, output, which are
           intended to provide information on the number of primer pairs that Primer3 examined,
           and statistics on the number discarded for various reasons. Default value: N

       -numreturn integer
           The maximum number of primer pairs to return. Primer pairs returned are sorted by
           their 'quality', in other words by the value of the objective function (where a lower
           number indicates a better primer pair). Caution: setting this parameter to a large
           value will increase running time. Default value: 5

       -outfile outfile


       Bugs can be reported to the Debian Bug Tracking system (, or
       directly to the EMBOSS developers


       primers is fully documented via the tfm(1) system.


       Debian Med Packaging Team <>
           Wrote the script used to autogenerate this manual page.


       This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package.
       It can be redistributed under the same terms as EMBOSS itself.