NAME

       tradis_essentiality  - bio-tradis: produces calls of gene essentiality using an adaptation
       of the method described in Langridge et al.

SYNOPSIS

       tradis_essentiality data.tab

DESCRIPTION

       Produces calls of gene essentiality  using  an  adaptation  of  the  method  described  in
       Langridge  et al. Genome Research 2009 and Barquist et al.  NAR 2013. A loess curve is fit
       to the distribution of insertion indices, and used to  identify  the  minima  between  the
       'essential'  and  'non-essential'  distributions. These distributions are then used to fit
       gamma distributions, which are then used to calculate log-odds ratios, which are  used  to
       determine  an  insertion-index  threshold  for  gene essentiality. Note that this analysis
       requires a saturated mutant library, and is not suitable for the  analysis  of  data  sets
       with  low insertion density. The script produces a number of diagnostic plots which can be
       used to verify that this condition has been met.

AUTHOR

       This manpage was written by Andreas Tille for the Debian distribution and can be used  for
       any other usage of the program.