Provided by: libbio-perl-perl_1.7.6-3_all bug


       Bio::Matrix::PSM::Psm - handle combination of site matricies


         use Bio::Matrix::PSM::IO;

         #To get a Psm object from a file use the Psm parser:
         my $psmIO =  Bio::Matrix::PSM::IO->new(-format=>'meme', -file=>$file);

         # Now go through all entities in the file with next_psm, which
         # returns a Psm object see Bio::Matrix::PSM::IO for detailed
         # documentation (matrix predictions or matrix sequence matches or
         # both):

         while (my $psm=$psmIO->next_psm) {
           my %psm_header=$psm->header;
           my $ic=$psm_header{IC};
           my $sites=$psm_header{sites};
           my $width=$psm_header{width};
           my $score=$psm_header{e_val};
           my $IUPAC=$psm->IUPAC;
           my $instances=$psm->instances;
           foreach my $instance (@{$instances}) {
             my $id=$instance->primary_id;
             #Do something with the id

        #or create from memmory:
         my $psm= Bio::Matrix::PSM::Psm->new( -pA=>\@pA,-pC=>\@pC,-pG=>\@pG,-pT=>\@pT,
              -instances=>$instances, -e_val=>$e_val,
              -IC=>$ic, -width=>$width, -sites=>$sites)

         # where pA through pG are the respective frequencies of the matrix (see also
         # Bio::Matrix::PSM::SiteMatrix), and everything else is self-explenatory,
         # except for -instances (reference to an array of
         #  Bio::Matrix::PSM::InstanceSite objects) which is documented below.


       To handle a combination of site matrices and/or their corresponding sequence matches
       (instances). This object inherits from Bio::Matrix::PSM::SiteMatrix, so you can use the
       respective methods. It may hold also an array of Bio::Matrix::PSM::InstanceSite object,
       but you will have to retrieve these through Bio::Matrix::PSM::Psm->instances method (see
       below). To some extent this is an expanded SiteMatrix object, holding data from analysis
       that also deal with sequence matches of a particular matrix.

       This does not make too much sense to me I am mixing PSM with PSM sequence matches Though
       they are very closely related, I am not satisfied by the way this is implemented here.
       Heikki suggested different objects when one has something like meme But does this mean we
       have to write a different objects for mast, meme, transfac, theiresias, etc.?  To me the
       best way is to return SiteMatrix object + arrray of InstanceSite objects and then mast
       will return undef for SiteMatrix and transfac will return undef for InstanceSite. Probably
       I cannot see some other design issues that might arise from such approach, but it seems
       more straightforward.  Hilmar does not like this because it is an exception from the
       general BioPerl rules Should I leave this as an option?  Also the header rightfully
       belongs the driver object, and could be retrieved as hashes.  I do not think it can be
       done any other way, unless we want to create even one more object with very unclear


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AUTHOR - Stefan Kirov



       This software is provided "as is" without warranty of any kind.


       SiteMatrix, meme, transfac, InstanceSite


        Title   : new
        Usage   : my $psm= Bio::Matrix::PSM::Psm->new( -pA=>\@pA,-pC=>\@pC,
                                                      -IC=>$ic, -width=>$width,
        Function: Creates a new Bio::Matrix::PSM::Psm object
        Throws  :
        Example :
        Returns :  Bio::Matrix::PSM::Psm object
        Args    :  hash

        Title   : instances
        Usage   :   my @instances=@{$psm->instances};
        Function: Gets/sets the instances (Bio::Matrix::PSM::InstanceSite objects)
                   associated with the Psm object
        Throws  :
        Example :
        Returns :  array reference (Bio::Matrix::PSM::InstanceSite objects)
        Args    :  array reference (Bio::Matrix::PSM::InstanceSite objects)

        Title   : header
        Usage   :  my %header=$psm->header;
                   my $ic=$psm->header('IC');
        Function: Gets the general information, common for most files,
                  dealing with PSM such as information content (IC), score
                  (e-value, etc.), number of sites (sites) and width. This
                  list may expand. The current list should be in
                  @Bio::Matrix::PSM::Psm::HEADER. Returns undef if an
                  argument is supplied that is not in
        Throws  :
        Example :
        Returns :  hash or string
        Args    :  string (IC, e_val...)

        Title   :  matrix
        Usage   :  my $matrix=$psm->matrix;
        Function:  Gets/sets the SiteMatrix related information
        Throws  :
        Example :
        Returns :  Bio::Matrix::PSM::SiteMatrix objects
        Args    :  Bio::Matrix::PSM::SiteMatrix objects