Provided by: libbio-perl-perl_1.7.2-3_all bug


       Bio::Tools::Analysis::Protein::GOR4 - a wrapper around GOR4 protein secondary structure
       prediction server


         use Bio::Tools::Analysis::Protein::GOR4;
         #get a Bio::Seq or Bio::PrimarySeq
         use Bio::PrimarySeq;
         $seq = Bio::PrimarySeq->new
            -primary_id=>'test'); # a Bio::PrimarySeqI object

         my $gor4 = Bio::Tools::Analysis::Protein::GOR4->new (-seq=>$seq);
         print $gor4->result;# #raw text to standard error


       A module to remotely retrieve predictions of protein secondary structure.  Each residue in
       the protein receives a score representing the likelihood of existing in each of three
       different states (helix, coil or sheet), e.g.,

         my $analysis_object = Bio::Tools::SimpleAnalysis::Protein::GOR4->
             new(-seq => $seq);

       creates a new object


       submits the query to the server and obtains raw text output

       Given an amino acid sequence the results can be obtained in 4 formats, determined by the
       argument to the result method

       1.  The raw text of the program output

             my $rawdata = $analysis_object->result;

       2.  An reference to an array of hashes of scores for each state and the assigned state.

             my $data_ref = $analysis_object->result('parsed');
             print "score for helix at residue 2 is $data_ref->[1]{'helix'}\n";
             print "predicted struc  at residue 2 is $data_ref->[1]{'struc}\n";

       3.  An array of Bio::SeqFeature::Generic objects where each feature is a predicted unit of
           secondary structure. Only stretches of helix/sheet predictions for longer than 4
           residues are defined as helices. See for examples of
           how to add sequence features.

             my @fts = $analysis_object->result(Bio::SeqFeatureI);
             for my $ft (@fts) {
                 print " From ",  $ft->start, " to  ",$ft->end, " struc: " ,
                        ($ft->each_tag_value('type'))[0]  ,"\n";

       4.  A Bio::Seq::Meta::Array implementing sequence.

           This is a Bio::Seq object that can also hold data about each residue in the sequence
           In this case, the sequence can be associated with a single array of GOR4 prediction
           scores.  e.g.,

             my $meta_sequence = $analysis_object->result('all');
             print "helix scores from residues 10-20 are ",
                 $meta_sequence->named_submeta_text("GOR4_helix",10,20), "\n";

           Meta sequence names are : GOR4_helix, GOR4_sheet, GOR4_coil, GOR4_struc, representing
           the scores for each residue.

           Many methods common to all analyses are inherited from


       Bio::SimpleAnalysisI, Bio::Tools::Analysis::SimpleAnalysisBase, Bio::Seq::Meta::Array,


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       The rest of the documentation details each of the object methods. Internal methods are
       usually preceded with a _

        Name    : result
        Usage   : $job->result (...)
        Returns : a result created by running an analysis
        Args    : see keys of $RESULT_SPEC

       The method returns a result of an executed job. If the job was terminated by an error the
       result may contain an error message instead of the real data.

       This implementation returns differently processed data depending on argument:

          Returns the raw ASCII data stream but without HTML tags

          The argument string defines the type of bioperl objects returned in an array.  The
          objects are Bio::SeqFeature::Generic.  Feature primary tag is "2ary".  Feature tags are
          "type" (which can be helix, sheet or coil) "method" (GOR4).

          Array of hash references of { helix =>, sheet => , coil => , struc=>}.

          A Bio::Seq::Meta::Array object. Scores can be accessed using methods from this class.
          Meta sequence names are GOR4_helix, GOR4_sheet, GOR4_coil, GOR4_struc.