Provided by: bioperl_1.6.901-2_all bug

NAME -- Genbank->gbrowse-friendly GFF3

 [options] filename(s)

         # process a directory containing GenBank flatfiles
         perl --dir path_to_files --zip

         # process a single file, ignore explicit exons and introns
         perl --filter exon --filter intron file.gbk.gz

         # process a list of files
         perl *gbk.gz

         # process data from URL, with Chado GFF model (-noCDS), and pipe to database loader
         curl \
         | perl -noCDS -in stdin -out stdout \
         | perl -dbname mychado -organism fromdata

               --noinfer  -r  don't infer exon/mRNA subfeatures
               --conf     -i  path to the curation configuration file that contains user preferences
                              for Genbank entries (must be YAML format)
                              (if --manual is passed without --ini, user will be prompted to
                               create the file if any manual input is saved)
               --sofile  -l  path to to the so.obo file to use for feature type mapping
                              (--sofile live will download the latest online revision)
               --manual   -m  when trying to guess the proper SO term, if more than
                              one option matches the primary tag, the converter will
                              wait for user input to choose the correct one
                              (only works with --sofile)
               --dir      -d  path to a list of genbank flatfiles
               --outdir   -o  location to write GFF files (can be 'stdout' or '-' for pipe)
               --zip      -z  compress GFF3 output files with gzip
               --summary  -s  print a summary of the features in each contig
               --filter   -x  genbank feature type(s) to ignore
               --split    -y  split output to separate GFF and fasta files for
                              each genbank record
               --nolump   -n  separate file for each reference sequence
                              (default is to lump all records together into one
                              output file for each input file)
               --ethresh  -e  error threshold for unflattener
                              set this high (>2) to ignore all unflattener errors
               --[no]CDS  -c  Keep CDS-exons, or convert to alternate gene-RNA-protein-exon
                              model. --CDS is default. Use --CDS to keep default GFF gene model,
                              use --noCDS to convert to g-r-p-e.
               --format   -f  Input format (SeqIO types): GenBank, Swiss or Uniprot, EMBL work
                              (GenBank is default)
               --GFF_VERSION  3 is default, 2 and 2.5 and other Bio::Tools::GFF versions available
               --quiet        don't talk about what is being processed
               --typesource   SO sequence type for source (e.g. chromosome; region; contig)
               --help     -h  display this message


       This script uses Bio::SeqFeature::Tools::Unflattener and Bio::Tools::GFF to convert
       GenBank flatfiles to GFF3 with gene containment hierarchies mapped for optimal display in

       The input files are assumed to be gzipped GenBank flatfiles for refseq contigs.  The files
       may contain multiple GenBank records.  Either a single file or an entire directory can be
       processed.  By default, the DNA sequence is embedded in the GFF but it can be saved into
       separate fasta file with the --split(-y) option.

       If an input file contains multiple records, the default behaviour is to dump all GFF and
       sequence to a file of the same name (with .gff appended).  Using the 'nolump' option will
       create a separate file for each genbank record.  Using the 'split' option will create
       separate GFF and Fasta files for each genbank record.

       'split' and 'nolump' produce many files

       In cases where the input files contain many GenBank records (for example, the chromosome
       files for the mouse genome build), a very large number of output files will be produced if
       the 'split' or 'nolump' options are selected.  If you do have lists of files > 6000, use
       the --long_list option in or to load the gff and/
       or fasta files.

       Designed for RefSeq

       This script is designed for RefSeq genomic sequence entries.  It may work for third party
       annotations but this has not been tested.  But see below, Uniprot/Swissprot works, EMBL
       and possibly EMBL/Ensembl if you don't mind some gene model unflattener errors (dgg).

       G-R-P-E Gene Model

       Don Gilbert worked this over with needs to produce GFF3 suited to loading to GMOD Chado
       databases.  Most of the changes I believe are suited for general use.  One main chado-
       specific addition is the
         --[no]cds2protein  flag

       My favorite GFF is to set the above as ON by default (disable with --nocds2prot) For
       general use it probably should be OFF, enabled with --cds2prot.

       This writes GFF with an alternate, but useful Gene model, instead of the consensus model
       for GFF3

         [ gene > mRNA> (exon,CDS,UTR) ]

       This alternate is

         gene > mRNA > polypeptide > exon

       means the only feature with dna bases is the exon.  The others specify only location
       ranges on a genome.  Exon of course is a child of mRNA and protein/peptide.

       The protein/polypeptide feature is an important one, having all the annotations of the
       GenBank CDS feature, protein ID, translation, GO terms, Dbxrefs to other proteins.

       UTRs, introns, CDS-exons are all inferred from the primary exon bases inside/outside
       appropriate higher feature ranges.   Other special gene model features remain the same.

       Several other improvements and bugfixes, minor but useful are included

         * IO pipes now work:
           curl ftp://ncbigenomes/... | genbank2gff3 --in stdin --out stdout | gff2chado ...

         * GenBank main record fields are added to source feature, e.g. organism, date,
           and the sourcetype, commonly chromosome for  genomes, is used.

         * Gene Model handling for ncRNA, pseudogenes are added.

         * GFF header is cleaner, more informative.
           --GFF_VERSION flag allows choice of v2 as well as default v3

         * GFF ##FASTA inclusion is improved, and
           CDS translation sequence is moved to FASTA records.

         * FT -> GFF attribute mapping is improved.

         * --format choice of SeqIO input formats (GenBank default).
           Uniprot/Swissprot and EMBL work and produce useful GFF.

         * SeqFeature::Tools::TypeMapper has a few FT -> SOFA additions
             and more flexible usage.


   Are these additions desired?
        * filter input records by taxon (e.g. keep only organism=xxx or taxa level = classYYY
        * handle Entrezgene, other non-sequence SeqIO structures (really should change
           those parsers to produce consistent annotation tags).

   Related bugfixes/tests
       These items from Bioperl mail were tested (sample data generating errors), and found

        From: Ed Green <green <at>>
        Subject: on new human RefSeq
        Date: 2006-03-13 21:22:26 GMT
          -- unspecified errors (sample data works now).

        From: Eric Just <e-just <at>>
        Date: 2007-01-26 17:08:49 GMT
          -- bug fixed in genbank2gff3 for multi-record handling

       This error is for a /trans_splice gene that is hard to handle, and unflattner/genbank2

        From: Chad Matsalla <chad <at>>
        Subject: genbank2gff3.PLS and the unflatenner - Inconsistent   order?
        Date: 2005-07-15 19:51:48 GMT


       Sheldon McKay (

       Copyright (c) 2004 Cold Spring Harbor Laboratory.

   AUTHOR of hacks for GFF2Chado loading
       Don Gilbert (