Provided by: libbio-perl-perl_1.6.901-2_all bug


       Bio::Tools::Phylo::PAML - Parses output from the PAML programs codeml, baseml, basemlg,
       codemlsites and yn00


         #!/usr/bin/perl -Tw
         use strict;

         use Bio::Tools::Phylo::PAML;

         # need to specify the output file name (or a fh) (defaults to
         # -file => "codeml.mlc"); also, optionally, the directory in which
         # the other result files (rst, 2ML.dS, etc) may be found (defaults
         # to "./")
         my $parser = Bio::Tools::Phylo::PAML->new
           (-file => "./results/mlc", -dir => "./results/");

         # get the first/next result; a Bio::Tools::Phylo::PAML::Result object,
         # which isa Bio::SeqAnalysisResultI object.
         my $result = $parser->next_result();

         # get the sequences used in the analysis; returns Bio::PrimarySeq
         # objects (OTU = Operational Taxonomic Unit).
         my @otus = $result->get_seqs();

         # codon summary: codon usage of each sequence [ arrayref of {
         # hashref of counts for each codon } for each sequence and the
         # overall sum ], and positional nucleotide distribution [ arrayref
         # of { hashref of frequencies for each nucleotide } for each
         # sequence and overall frequencies ]:
         my ($codonusage, $ntdist) = $result->get_codon_summary();

         # example manipulations of $codonusage and $ntdist:
         printf "There were %d %s codons in the first seq (%s)\n",
           $codonusage->[0]->{AAA}, 'AAA', $otus[0]->id();
         printf "There were %d %s codons used in all the sequences\n",
           $codonusage->[$#{$codonusage}]->{AAA}, 'AAA';
         printf "Nucleotide %c was present %g of the time in seq %s\n",
           'A', $ntdist->[1]->{A}, $otus[1]->id();

         # get Nei & Gojobori dN/dS matrix:
         my $NGmatrix = $result->get_NGmatrix();

         # get ML-estimated dN/dS matrix, if calculated; this corresponds to
         # the runmode = -2, pairwise comparison usage of codeml
         my $MLmatrix = $result->get_MLmatrix();

         # These matrices are length(@otu) x length(@otu) "strict lower
         # triangle" 2D-matrices, which means that the diagonal and
         # everything above it is undefined.  Each of the defined cells is a
         # hashref of estimates for "dN", "dS", "omega" (dN/dS ratio), "t",
         # "S" and "N".  If a ML matrix, "lnL" and "kappa" will also be defined.
         printf "The omega ratio for sequences %s vs %s was: %g\n",
           $otus[0]->id, $otus[1]->id, $MLmatrix->[0]->[1]->{omega};

         # with a little work, these matrices could also be passed to
         # Bio::Tools::Run::Phylip::Neighbor, or other similar tree-building
         # method that accepts a matrix of "distances" (using the LOWTRI
         # option):
         my $distmat = [ map { [ map { $$_{omega} } @$_ ] } @$MLmatrix ];

         # for runmode's other than -2, get tree topology with estimated
         # branch lengths; returns a Bio::Tree::TreeI-based tree object with
         # added PAML parameters at each node
         my ($tree) = $result->get_trees();
         for my $node ($tree->get_nodes()) {
            # inspect the tree: the "t" (time) parameter is available via
            # $node->branch_length(); all other branch-specific parameters
            # ("omega", "dN", etc.) are available via
            # ($omega) = $node->get_tag_values('omega');

         # if you are using model based Codeml then trees are stored in each
         # modelresult object
         for my $modelresult ( $result->get_NSSite_results ) {
           # model M0, M1, etc
           print "model is ", $modelresult->model_num, "\n";
           my ($tree) = $modelresult->get_trees();
           for my $node ($tree->get_nodes()) {
            # inspect the tree: the "t" (time) parameter is available via
            # $node->branch_length(); all other branch-specific parameters
            # ("omega", "dN", etc.) are available via
            # ($omega) = $node->get_tag_values('omega');

         # get any general model parameters: kappa (the
         # transition/transversion ratio), NSsites model parameters ("p0",
         # "p1", "w0", "w1", etc.), etc.
         my $params = $result->get_model_params();
         printf "M1 params: p0 = %g\tp1 = %g\n", $params->{p0}, $params->{p1};

         # parse AAML result files
         my $aamat = $result->get_AADistMatrix();
         my $aaMLmat = $result->get_AAMLDistMatrix();


       This module is used to parse the output from the PAML programs codeml, baseml, basemlg,
       codemlsites and yn00.  You can use the Bio::Tools::Run::Phylo::PAML::* modules to actually
       run some of the PAML programs, but this module is only useful to parse the output.

       This module has fledgling support for PAML version 4.3a (October 2009).  Please report any
       problems to the mailing list (see FEEDBACK below).


       Implement get_posteriors(). For NSsites models, obtain arrayrefs of posterior
       probabilities for membership in each class for every position; probabilities correspond to
       classes w0, w1, ... etc.

         my @probs = $result->get_posteriors();

         # find, say, positively selected sites!
         if ($params->{w2} > 1) {
           for (my $i = 0; $i < @probs ; $i++) {
             if ($probs[$i]->[2] > 0.5) {
                # assumes model M1: three w's, w0, w1 and w2 (positive selection)
                printf "position %d: (%g prob, %g omega, %g mean w)\n",
                  $i, $probs[$i]->[2], $params->{w2}, $probs[$i]->[3];
         } else { print "No positive selection found!\n"; }


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AUTHOR - Jason Stajich, Aaron Mackey

       Email Email


       Albert Vilella avilella-AT-gmail-DOT-com Sendu Bala Dave Messina


       RST parsing -- done, Avilella contributions bug#1506, added by jason 1.29
                   -- still need to parse in joint probability and non-syn changes
                      at site table


       The rest of the documentation details each of the object methods.  Internal methods are
       usually preceded with a _

        Title   : new
        Usage   : my $obj = Bio::Tools::Phylo::PAML->new(%args);
        Function: Builds a new Bio::Tools::Phylo::PAML object
        Returns : Bio::Tools::Phylo::PAML
        Args    : Hash of options: -file, -fh, -dir
                  -file (or -fh) should contain the contents of the PAML
                  -dir is the (optional) name of the directory in
                       which the PAML program was run (and includes other
                       PAML-generated files from which we can try to gather data)

   Implement Bio::AnalysisParserI interface
        Title   : next_result
        Usage   : $result = $obj->next_result();
        Function: Returns the next result available from the input, or
                  undef if there are no more results.
        Example :
        Returns : a Bio::Tools::Phylo::PAML::Result object
        Args    : none