Provided by: libbio-perl-perl_1.6.901-2_all bug


       Bio::Tools::SiRNA::Ruleset::tuschl - Perl object implementing the tuschl group's rules for
       designing small inhibitory RNAs


       Do not use this module directly.  Instead, use Bio::Tools::SiRNA and specify the tuschl

         use Bio::Tools::SiRNA;

         my $sirna_designer = Bio::Tools::SiRNA->new( -target => $bio_seq,
                                                      -rules  => 'tuschl'
         my @pairs = $sirna_designer->design;

         foreach $pair (@pairs) {
             my $sense_oligo_sequence = $pair->sense->seq;
             my $antisense_oligo_sequence = $pair->antisense->seq;

             # print out results
             print join ("\t", $pair->start, $pair->end, $pair->rank,
                         $sense_oligo_sequence, $antisense_oligo_sequence), "\n";


       This package implements the rules for designing siRNA reagents developed by Tuschl and
       colleagues (see It looks for
       oligos that match the following patterns in the target sequence:

         1. AA(N19)TT (rank 1)
         2. AA(N21) (rank 2)
         3. NA(N21) (rank 3)

       The package also supports selection of siRNA seqences that can be transcribed by pol3:



       Bio::Tools::SiRNA, Bio::SeqFeature::SiRNA::Pair, Bio::SeqFeature::SiRNA::Oligo.


   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send
       your comments and suggestions preferably to the Bioperl mailing list.  Your participation
       is much appreciated.
                  - General discussion  - About the mailing lists

       Please direct usage questions or support issues to the mailing list:

       rather than to the module maintainer directly. Many experienced and reponsive experts will
       be able look at the problem and quickly address it. Please include a thorough description
       of the problem with code and data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their
       resolution. Bug reports can be submitted via the web:


       Donald Jackson (


       The rest of the documentation details each of the object methods.  Internal methods are
       usually preceded with a _

         Title : new
         Usage : Do not call directly - use Bio::Tools::SiRNA->new instead.
         Returns : Bio::Tools::SiRNA::Ruleset::saigo object
         Args  : none