NAME

       andi - estimates evolutionary distance

SYNOPSIS

       andi [-jlv] [-b INT] [-p FLOAT] [-m MODEL] [-t INT] FILES...

DESCRIPTION

       andi  estimates  the  evolutionary distance between closely related genomes. For this andi
       reads the input sequences from FASTA files and computes the pairwise anchor distance.  The
       idea behind this is explained in a paper by Haubold et al. (see below).

OUTPUT

       The output is a symmetrical distance matrix in PHYLIP format, with each entry representing
       divergence with a positive real number. A distance of zero means that  two  sequences  are
       identical, whereas other values are estimates for the nucleotide substitution rate (Jukes-
       Cantor corrected). For technical reasons the comparison might fail and no estimate can  be
       computed.  In  such  cases nan is printed. This either means that the input sequences were
       too short (<200bp) or too diverse (K>0.5) for our method to work properly.

OPTIONS

       -b, --bootstrap <INT>
              Compute multiple distance matrices, with n-1 bootstrapped from the first.  See  the
              paper Klötzl & Haubold (2016, in review) for a detailed explanation.

       -j, --join
              Use  this  mode  if  each of your FASTA files represents one assembly with numerous
              contigs. andi will then treat all of the contained sequences per file as  a  single
              genome.  In  this  mode  at  least  one  filename must be provided via command line
              arguments. For the output the filename is used to identify each sequence.

       -l, --low-memory
              In multithreaded mode, andi requires memory linear to the amount  of  threads.  The
              low  memory mode changes this to a constant demand independent from the used number
              of threads. Unfortunately, this comes at a significant runtime cost.

       -m, --model <Raw|JC|Kimura>
              Different models of nucleotide evolution are supported. By default the Jukes-Cantor
              correction is used.

       -p <FLOAT>
              Significance of an anchor pair; default: 0.05.

       -t <INT>
              The number of threads to be used; by default, all available processors are used.
              Multithreading is only available if andi was compiled with OpenMP support.

       -v, --verbose
              Prints additional information. Apply multiple times for extra verboseness.

       -h, --help
              Prints the synopsis and an explanation of available options.

       --version
              Outputs version information and acknowledgments.

COPYRIGHT

       Copyright © 2014, 2015 Fabian Klötzl License GPLv3+: GNU GPL version 3 or later.
       This  is free software: you are free to change and redistribute it.  There is NO WARRANTY,
       to  the  extent  permitted  by   law.    The   full   license   text   is   available   at
       <http://gnu.org/licenses/gpl.html>.

ACKNOWLEDGMENTS

       1)  andi:  Haubold,  B.  Klötzl,  F.  and Pfaffelhuber, P. (2015). andi: Fast and accurate
       estimation of evolutionary distances between closely related genomes
       2) Algorithms: Ohlebusch, E. (2013). Bioinformatics Algorithms. Sequence Analysis,  Genome
       Rearrangements, and Phylogenetic Reconstruction. pp 118f.
       3)  SA  construction:  Mori,  Y.  (2005).  Short  description of improved two-stage suffix
       sorting algorithm. http://homepage3.nifty.com/wpage/software/itssort.txt

BUGS

   Reporting Bugs
       Please report bugs to <kloetzl@evolbio.mpg.de> or at <https://github.com/EvolBioInf/andi>.