bionic (3) Bio::Restriction::Enzyme::MultiCut.3pm.gz

Provided by: libbio-perl-perl_1.7.2-2_all bug

NAME
       Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease


SYNOPSIS
         # set up a single restriction enzyme. This contains lots of
         # information about the enzyme that is generally parsed from a
         # rebase file and can then be read back

         use Bio::Restriction::Enzyme;


DESCRIPTION
       This module defines a restriction endonuclease class where one object represents one of the distinct
       recognition sites for that enzyme. The method others stores references to other objects with alternative
       sites.

       In this schema each object within an EnzymeCollection can be checked for matching a sequence.

       REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means:

          Bsp24I
                    5'      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3'
                    3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5'


FEEDBACK
   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments
       and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated.

         bioperl-l@bioperl.org                  - General discussion
         http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and reponsive experts will be able look
       at the problem and quickly address it. Please include a thorough description of the problem with code and
       data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug
       reports can be submitted via the web:

         https://github.com/bioperl/bioperl-live/issues


AUTHOR
       Heikki Lehvaslaiho, heikki-at-bioperl-dot-org


CONTRIBUTORS
       Rob Edwards, redwards@utmem.edu


       Copyright (c) 2003 Rob Edwards.

       Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved.  This module is free
       software; you can redistribute it and/or modify it under the same terms as Perl itself.


SEE ALSO
       Bio::Restriction::Enzyme, Bio::Restriction::Analysis, Bio::Restriction::EnzymeCollection


APPENDIX
       Methods beginning with a leading underscore are considered private and are intended for internal use by
       this module. They are not considered part of the public interface and are described here for
       documentation purposes only.

   new
        Title     : new
        Function
        Function  : Initializes the enzyme object
        Returns   : The Restriction::Enzyme::MultiCut object
        Argument  :

   others
        Title     : others
        Usage     : $re->others(@enz_obj_array);
        Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
        Arguments : optional array of Enzyme::MultiCut objects
        Returns   : array of Enzyme objects

       Added for compatibility to REBASE

   purge_others
        Title     : purge_others
        Usage     : $re->purge_references();
        Function  : Purges the set of references for this enzyme
        Arguments :
        Returns   :