Provided by: subread_1.6.0+dfsg-1_amd64 
NAME
subread-align - an accurate and efficient aligner for mapping both genomic DNA-seq reads and RNA-seq
reads (for the purpose of expression analysis)
USAGE
subread-align [options] -i <index_name> -r <input> -t <type> -o <output>
## Mandatory arguments:
-i <string>
Base name of the index.
-r <string>
Name of an input read file. If paired-end, this should be the first read file (typically
containing "R1"in the file name) and the second should be provided via "-R". Acceptable formats
include gzipped FASTQ, FASTQ and FASTA. These formats are identified automatically.
-t <int>
Type of input sequencing data. Its values include 0: RNA-seq data 1: genomic DNA-seq data.
## Optional arguments: # input reads and output
-o <string>
Name of an output file. By default, the output is in BAM format. Omitting this option makes the
output be written to STDOUT.
-R <string>
Name of the second read file in paired-end data (typically containing "R2" the file name).
--SAMinput
Input reads are in SAM format.
--BAMinput
Input reads are in BAM format.
--SAMoutput
Save mapping results in SAM format.
# Phred offset
-P <3:6>
Offset value added to the Phred quality score of each read base. '3' for phred+33 and '6' for
phred+64. '3' by default.
# thresholds for mapping
-n <int>
Number of selected subreads, 10 by default.
-m <int>
Consensus threshold for reporting a hit (minimal number of subreads that map in consensus) . If
paired-end, this gives the consensus threshold for the anchor read (anchor read receives more
votes than the other read in the same pair). 3 by default
-p <int>
Consensus threshold for the non- anchor read in a pair. 1 by default.
-M <int>
Maximum number of mis-matched bases allowed in each reported alignment. 3 by default. Mis-matched
bases found in softclipped bases are not counted.
# unique mapping and multi-mapping
--multiMapping
Report multi-mapping reads in addition to uniquely mapped reads. Use "-B" to set the maximum
number of equally-best alignments to be reported.
-B <int>
Maximum number of equally-best alignments to be reported for a multi-mapping read. Equally-best
alignments have the same number of mis-matched bases. 1 by default.
# indel detection
-I <int>
Maximum length (in bp) of indels that can be detected. 5 by default. Indels of up to 200bp long
can be detected.
--complexIndels
Detect multiple short indels that are in close proximity (they can be as close as 1bp apart from
each other).
# read trimming
--trim5 <int>
Trim off <int> number of bases from 5' end of each read. 0 by default.
--trim3 <int>
Trim off <int> number of bases from 3' end of each read. 0 by default.
# distance and orientation of paired end reads
-d <int>
Minimum fragment/insert length, 50bp by default.
-D <int>
Maximum fragment/insert length, 600bp by default.
-S <ff:fr:rf>
Orientation of first and second reads, 'fr' by default ( forward/reverse).
# number of CPU threads
-T <int>
Number of CPU threads used, 1 by default.
# read group
--rg-id <string>
Add read group ID to the output.
--rg <string>
Add <tag:value> to the read group (RG) header in the output.
# color space reads
-b Convert color-space read bases to base-space read bases in the mapping output. Note that read
mapping is performed at color-space.
# dynamic programming
--DPGapOpen <int> Penalty for gap opening in short indel detection. -1 by
default.
--DPGapExt <int>
Penalty for gap extension in short indel detection. 0 by default.
--DPMismatch <int> Penalty for mismatches in short indel detection. 0 by
default.
--DPMatch <int>
Score for matched bases in short indel detection. 2 by default.
# detect structural variants
--sv Detect structural variants (eg. long indel, inversion, duplication and translocation) and report
breakpoints. Refer to Users Guide for breakpoint reporting.
# gene annotation
-a Name of an annotation file. GTF/GFF format by default. See -F option for more format information.
-F Specify format of the provided annotation file. Acceptable formats include 'GTF' (or compatible
GFF format) and 'SAF'. 'GTF' by default. For SAF format, please refer to Users Guide.
-A Provide a chromosome name alias file to match chr names in annotation with those in the reads.
This should be a twocolumn comma-delimited text file. Its first column should include chr names in
the annotation and its second column should include chr names in the index. Chr names are case
sensitive. No column header should be included in the file.
--gtfFeature <string>
Specify feature type in GTF annotation. 'exon' by default. Features used for read counting will be
extracted from annotation using the provided value.
--gtfAttr <string>
Specify attribute type in GTF annotation. 'gene_id' by default. Meta-features used for read
counting will be extracted from annotation using the provided value.
# others
-v Output version of the program.
Refer to Users Manual for detailed description to the arguments.