Provided by: gbrowse_2.56+dfsg-3build1_all 
NAME
Bio::Graphics::Browser2::PadAlignment - Insert pads into a multiple alignment
VERSION (CVS-info)
$RCSfile: PadAlignment.pm,v $
$Revision$
$Author: lstein $
$Date: 2008-09-09 20:53:36 $
SYNOPSIS
use Bio::Graphics::Browser2::PadAlignment;
my @dnas = (
dna1 =>'FFFFgatcGATCgatcGATCgatcGATCgatcGBATCgatcGATCatcGATCgatcGATCgatcGATCgatcGATgatcGATCgatcNNNNGATC',
dna2 =>'FFgatcGATCGATCgatcNNGATCgatcGATCgatcGATCgatcGATCgatcGATCtcGATBCgatcGATCatcGATCgatcNNNNGATCFFFF',
dna3 =>'FFFFgatcGATCgatcGATCgatcGATCgatcBBBGATCgatcGATCatcGATCBgatcGATCgatcGATCgatcGATgatcGATCgatcNNNNGATCFF',
dna4 =>'ZZFFFFgatcGATCgatcGATCgatcGATCgatc',
dna5 =>'ATBGGATtcttttttt',
);
# target st en tst ten
my @alignments = ([ 'dna2', 4, 11, 2, 9 ],
[ 'dna2', 16, 23, 10, 17 ],
[ 'dna2', 24, 32, 20, 28 ],
[ 'dna2', 34, 44, 29, 39 ],
[ 'dna2', 45, 59, 41, 55 ],
[ 'dna2', 62, 66, 56, 60 ],
[ 'dna2', 67, 74, 62, 69 ],
[ 'dna2', 76, 86, 71, 81 ],
[ 'dna2', 91, 94, 86, 89 ],
[ 'dna3', 4, 31, 4, 31 ],
[ 'dna3', 33, 33, 34, 34 ],
[ 'dna3', 34, 51, 36, 53 ],
[ 'dna3', 52, 86, 55, 89 ],
[ 'dna3', 91, 94, 94, 97 ],
[ 'dna4', 0, 31, 2, 33 ],
[ 'dna5', 17, 18, 0, 1 ],
[ 'dna5', 41, 43, 4, 6 ],
[ 'dna5', 85, 86, 7, 8 ],
);
my $align = Bio::Graphics::Browser2::PadAlignment->new(\@dnas,\@alignments);
my @padded = $align->padded_sequences;
print join "\n",@padded,"\n";
# ..FFFFgatcGATCgatcGATCgatc--GATCgatcG-B-ATCgatcGATC-atcGATC-gatcGATCgatcGAT-CgatcGATgatcGATCgatcNNNNGATC....
# ....FFgatcGATC----GATCgatcNNGATCgatcG---ATCgatcGATCgatcGATC-gatcGATC--tcGATBCgatcGATCatcGATCgatcNNNNGATCFFFF
# ..FFFFgatcGATCgatcGATCgatc--GATCgatcBBBGATCgatcGATC-atcGATCBgatcGATCgatcGAT-CgatcGATgatcGATCgatcNNNNGATCFF..
# ZZFFFFgatcGATCgatcGATCgatc--GATCgatc........................................................................
# ...................AT-----------------BG-------GAT--------------------------------------------tcttttttt.....
my $pretty = $align->alignment;
print $pretty,"\n";
# dna1 1 ..FFFFgatc GATCgatcGA TCgatc--GA TCgatcG-B- ATCgatcGAT C-atcGATC- gatcGATCga tcGAT-Cgat
# dna2 1 ....FFgatc GATC----GA TCgatcNNGA TCgatcG--- ATCgatcGAT CgatcGATC- gatcGATC-- tcGATBCgat
# dna3 1 ..FFFFgatc GATCgatcGA TCgatc--GA TCgatcBBBG ATCgatcGAT C-atcGATCB gatcGATCga tcGAT-Cgat
# dna4 1 ZZFFFFgatc GATCgatcGA TCgatc--GA TCgatc.... .......... .......... .......... ..........
# dna5 1 .......... .........A T--------- ---------- -----BGGAT ---------- ---------- ----------
# dna1 72 cGATgatcGA TCgatcNNNN GATC....
# dna2 67 cGATCatcGA TCgatcNNNN GATCFFFF
# dna3 75 cGATgatcGA TCgatcNNNN GATCFF..
# dna4 35 .......... .......... ........
# dna5 8 ---------- ----tctttt ttt.....
DESCRIPTION
This is a utility module for pretty-printing the type of alignment that comes out of gbrowse, namely a
multiple alignment in which each target is aligned to a reference genome without explicit pads or other
spaces.
For speed and ease of use, the module does not use form Bio::SeqI objects, but raw strings and alignment
data structures. This may change.
This module does not perform multiple alignments! It merely pretty-prints them!
METHODS
This section describes the methods used by this class.
$aligner = Bio::Graphics::Browser2::PadAlignment->new(\@sequences,\@alignments)
Create a new aligner. The two arguments are \@sequences, an array ref to the list of sequences to be
aligned, and \@alignments, an array ref describing how the sequences are to be aligned.
\@sequences should have the following structure:
[ name1 => $sequence1,
name2 => $sequence2,
name3 => $sequence3 ]
The sequences will be displayed in top to bottom order in the order provided. The first sequence in
the list is special because it is the reference sequence. All alignments are relative to it.
\@alignments should have the following structure:
[ [ target1, $start1, $end1, $tstart1, $tend1 ],
[ target1, $start2, $end2, $tstart2, $tend2 ],
...
]
Each element of @alignments is an arrayref with five elements. The first element is the name of the
target sequence, which must be one of the named sequences given in @sequences. The second and third
elements are the start and stop position of the aligned target segment relative to the reference
sequence, "name1" in the example given above. The fourth and fifth elements are the start and stop
position of the aligned target segment in the coordinate space of the target.
Example:
@dnas = ('dna1' => 'ccccccaaaaaatttt',
'dna2' => 'aaaaaa');
@alignment = ( ['dna2', 6, 11, 0, 5 ]);
Positions 0 to 5 of "dna2" align to positions 6-11 of "dna1".
Note that sequence positions are zero based. This may change.
@lines = $aligner->padded_sequences
This inserts pads into the sequences and returns them as a list of strings in the order specified in
new(). In a scalar context, this method will return a hashref in which the keys are the sequence
names and the values are their padded strings.
$map = $aligner->gap_map
This returns an arrayref indicating the position of each base in the gapped reference sequence. The
indexes are base positions, and the element values are their positions in the reference sequence as
returned by padded_sequences().
Note that the gap map only provides coordinate mapping for the reference sequence. For an
alternative implementation that provides gap maps for each of the targets (at the cost of speed and
memory efficiency) see the section after __END__ in the source file for this module.
$align_string = $aligner->alignment(\%origins [,\%options])
This method returns a pretty-printed string of the aligned sequences. You may provide a hashref of
sequence origins in order to control the numbers printed next to each line of the alignment. The
keys of the %origins hashref are the names of the sequences, and the values are the coordinate to be
assigned to the first base of the sequence. Use a negative number if you wish to indicate that the
sequence has been reverse complemented (the negative number should indicate the coordinate of the
first base in the provided sequence).
An optional second argument, if present, contains a hash reference to a set of option=>value pairs.
Three options are recognized:
show_mismatches 0|1 if true, highlight mismatches in pink
show_matches 0|1 if true, hightligt matches in yellow
color_code_proteins 0|1 if true, highlight amino acids thus:
Acidic amino acids in red
Basic amino acids in blue
Hydrophobic amino acids in grey
Polar amino acids in yellow
flip 0|1 if true, reverse complement the whole alignment
SEE ALSO
Bio::Graphics::Panel, Bio::Graphics::Glyph, Bio::Graphics::Feature, Bio::Graphics::FeatureFile,
Bio::Graphics::Browser, Bio::Graphics::Browser2::Plugin
AUTHOR
Lincoln Stein <lstein@cshl.org>.
Copyright (c) 2002 Cold Spring Harbor Laboratory
This library is free software; you can redistribute it and/or modify it under the same terms as Perl
itself. See DISCLAIMER.txt for disclaimers of warranty.