Provided by: libbio-graphics-perl_2.40-2_all 
NAME
Bio::Graphics::Glyph - Base class for Bio::Graphics::Glyph objects
SYNOPSIS
See Bio::Graphics::Panel.
DESCRIPTION
Bio::Graphics::Glyph is the base class for all glyph objects. Each glyph is a wrapper around an
Bio:SeqFeatureI object, knows how to render itself on an Bio::Graphics::Panel, and has a variety of
configuration variables.
End developers will not ordinarily work directly with Bio::Graphics::Glyph objects, but with
Bio::Graphics::Glyph::generic and its subclasses. Similarly, most glyph developers will want to subclass
from Bio::Graphics::Glyph::generic because the latter provides labeling and arrow-drawing facilities.
METHODS
This section describes the class and object methods for Bio::Graphics::Glyph.
CONSTRUCTORS
Bio::Graphics::Glyph objects are constructed automatically by an Bio::Graphics::Glyph::Factory, and are
not usually created by end-developer code.
$glyph = Bio::Graphics::Glyph->new(-feature=>$feature,-factory=>$factory)
Given a sequence feature, creates an Bio::Graphics::Glyph object to display it. The -feature
argument points to the Bio:SeqFeatureI object to display, and -factory indicates an
Bio::Graphics::Glyph::Factory object from which the glyph will fetch all its run-time configuration
information. Factories are created and manipulated by the Bio::Graphics::Panel object.
A standard set of options are recognized. See OPTIONS.
OBJECT METHODS
Once a glyph is created, it responds to a large number of methods. In this section, these methods are
grouped into related categories.
Retrieving glyph context:
$factory = $glyph->factory
Get the Bio::Graphics::Glyph::Factory associated with this object. This cannot be changed once it is
set.
$panel = $glyph->panel
Get the Bio::Graphics::Panel associated with this object. This cannot be changed once it is set.
$feature = $glyph->feature
Get the sequence feature associated with this object. This cannot be changed once it is set.
$feature = $glyph->parent_feature()
Within callbacks only, the parent_feature() method returns the parent of the current feature, if
there is one. Called with a numeric argument, ascends the parentage tree: parent_feature(1) will
return the parent, parent_feature(2) will return the grandparent, etc. If there is no parent, returns
undef.
$feature = $glyph->add_feature(@features)
Add the list of features to the glyph, creating subparts. This is most common done with the track
glyph returned by Bio::Graphics::Panel->add_track().
If the Bio::Graphics::Panel was initialized with -feature_limit set to a non-zero value, then calls
to a track glyph's add_feature() method will maintain a count of features added to the track. Once
the feature count exceeds the value set in -feature_limit, additional features will displace existing
ones in a way that effects a uniform sampling of the total feature set. This is useful to protect
against excessively large tracks. The total number of features added can be retrieved by calling the
glyph's feature_count() method.
$feature = $glyph->add_group(@features)
This is similar to add_feature(), but the list of features is treated as a group and can be
configured as a set.
$glyph->finished
When you are finished with a glyph, you can call its finished() method in order to break cycles that
would otherwise cause memory leaks. finished() is typically only used by the Panel object.
$subglyph = $glyph->make_subglyph($level,@sub_features)
This method is called to create subglyphs from a list of subfeatures. The $level indicates the
current level of the glyph (top-level glyphs are level 0, subglyphs are level 1, etc).
Ordinarily this method simply calls $self->factory->make_subglyph($level,@sub_features). Override it
in subclasses to create subglyphs of a particular type. For example:
sub make_subglyph {
my $self = shift;
my $level = shift;
my $factory = $self->factory;
$factory->make_glyph($factory,'arrow',@_);
}
$count = $glyph->feature_count()
Return the number of features added to this glyph via add_feature().
$flag = $glyph->features_clipped()
If the panel was initialized with -feature_limit set to a non-zero value, then calls to
add_features() will limit the number of glyphs to the indicated value. If this value was exceeded,
then features_clipped() will return true.
Retrieving glyph options:
$fgcolor = $glyph->fgcolor
$bgcolor = $glyph->bgcolor
$fontcolor = $glyph->fontcolor
$fontcolor = $glyph->font2color
$fillcolor = $glyph->fillcolor
These methods return the configured foreground, background, font, alternative font, and fill colors
for the glyph in the form of a GD::Image color index.
$color = $glyph->tkcolor
This method returns a color to be used to flood-fill the entire glyph before drawing (currently used
by the "track" glyph).
($left,$top,$right,$bottom) = $glyph->bounds($dx,$dy)
Given the topleft coordinates of the glyph, return the bounding box of its contents, exclusive of
padding. This is typically called by the draw() and draw_component() methods to recover the position
of the glyph.
($left,$top,$right,$bottom) = $glyph->calculate_boundaries($dx,$dy)
An alias for bounds(), used by some glyphs for compatibility with older versions of this module.
$width = $glyph->width([$newwidth])
Return the width of the glyph, not including left or right padding. This is ordinarily set
internally based on the size of the feature and the scale of the panel.
$width = $glyph->layout_width
Returns the width of the glyph including left and right padding.
$width = $glyph->height
Returns the height of the glyph, not including the top or bottom padding. This is calculated from
the "height" option and cannot be changed.
$font = $glyph->font
Return the font for the glyph.
$option = $glyph->option($option)
Return the value of the indicated option.
$index = $glyph->color($option_name)
Given an option name that corresponds to a color (e.g. 'fgcolor') look up the option and translate it
into a GD color index.
$index = $glyph->translate_color($color)
Given a symbolic or #RRGGBB-form color name, returns its GD index.
$level = $glyph->level
The "level" is the nesting level of the glyph. Groups are level -1, top level glyphs are level 0,
subparts (e.g. exons) are level 1 and so forth.
@parts = $glyph->parts
For glyphs that can contain subparts (e.g. the segments glyph), this method will return the list of
subglyphs it contains. Subglyphs are created automatically by the new() method and are created
subject to the maximum recursion depth specified by the maxdepth() method and/or the -maxdepth
option.
Setting an option:
$glyph->configure(-name=>$value)
You may change a glyph option after it is created using set_option(). This is most commonly used to
configure track glyphs.
Retrieving information about the sequence:
$start = $glyph->start
$end = $glyph->end
These methods return the start and end of the glyph in base pair units.
$offset = $glyph->offset
Returns the offset of the segment (the base pair at the far left of the image).
$length = $glyph->length
Returns the length of the sequence segment.
Retrieving formatting information:
$top = $glyph->top
$left = $glyph->left
$bottom = $glyph->bottom
$right = $glyph->right
These methods return the top, left, bottom and right of the glyph in pixel coordinates.
$height = $glyph->height
Returns the height of the glyph. This may be somewhat larger or smaller than the height suggested by
the GlyphFactory, depending on the type of the glyph.
$scale = $glyph->scale
Get the scale for the glyph in pixels/bp.
$height = $glyph->labelheight
Return the height of the label, if any.
$label = $glyph->label
Return a human-readable label for the glyph.
These methods are called by Bio::Graphics::Track during the layout process:
$glyph->move($dx,$dy)
Move the glyph in pixel coordinates by the indicated delta-x and delta-y values.
($x1,$y1,$x2,$y2) = $glyph->box
Return the current position of the glyph.
These methods are intended to be overridden in subclasses:
$glyph->calculate_height
Calculate the height of the glyph.
$glyph->calculate_left
Calculate the left side of the glyph.
$glyph->calculate_right
Calculate the right side of the glyph.
$glyph->draw($gd,$left,$top)
Optionally offset the glyph by the indicated amount and draw it onto the GD::Image object.
$glyph->draw_label($gd,$left,$top)
Draw the label for the glyph onto the provided GD::Image object, optionally offsetting by the amounts
indicated in $left and $right.
$glyph->maxdepth()
This returns the maximum number of levels of feature subparts that the glyph will recurse through.
For example, returning 0 indicates that the glyph will only draw the top-level feature. Returning 1
indicates that it will only draw the top-level feature and one level of subfeatures. Returning 2 will
descend down two levels. Overriding this method will speed up rendering by avoiding creating of a
bunch of subglyphs that will never be drawn.
The default behavior is to return undef (unlimited levels of descent) unless the -maxdepth option is
passed, in which case this number is returned.
Note that Bio::Graphics::Glyph::generic overrides maxdepth() to return 0, meaning no descent into
subparts will be performed.
These methods are useful utility routines:
@pixels = $glyph->map_pt(@bases);
Map the list of base position, given in base pair units, into pixels, using the current scale and
glyph position. This method will accept a single base position or an array.
$glyph->filled_box($gd,$x1,$y1,$x2,$y2)
Draw a filled rectangle with the appropriate foreground and fill colors, and pen width onto the
GD::Image object given by $gd, using the provided rectangle coordinates.
$glyph->filled_oval($gd,$x1,$y1,$x2,$y2)
As above, but draws an oval inscribed on the rectangle.
$glyph->exceeds_depth
Returns true if descending into another level of subfeatures will exceed the value returned by
maxdepth().
OPTIONS
The following options are standard among all Glyphs. See individual glyph pages for more options.
Also try out the glyph_help.pl script, which attempts to document each glyph's shared and specific
options and provides an interface for graphically inspecting the effect of different options.
Option Description Default
------ ----------- -------
-fgcolor Foreground color black
-bgcolor Background color turquoise
-fillcolor Synonym for -bgcolor
-linewidth Line width 1
-height Height of glyph 10
-font Glyph font gdSmallFont
-connector Connector type undef (false)
-connector_color
Connector color black
-strand_arrow Whether to indicate undef (false)
strandedness
-stranded Whether to indicate undef (false)
strandedness
(same as above))
-label Whether to draw a label undef (false)
-description Whether to draw a description undef (false)
-no_subparts Set to true to prevent undef (false)
drawing of the subparts
of a feature.
-ignore_sub_part Give the types/methods of undef
subparts to ignore (as a
space delimited list).
-maxdepth Specifies the maximum number undef (unlimited)
child-generations to decend
when getting subfeatures
-sort_order Specify layout sort order "default"
-always_sort Sort even when bumping is off undef (false)
-bump_limit Maximum number of levels to bump undef (unlimited)
-hilite Highlight color undef (no color)
-link, -title, -target
These options are used when creating imagemaps
for display on the web. See L<Bio::Graphics::Panel/"Creating Imagemaps">.
For glyphs that consist of multiple segments, the -connector option controls what's drawn between the
segments. The default is undef (no connector). Options include:
"hat" an upward-angling conector
"solid" a straight horizontal connector
"quill" a decorated line with small arrows indicating strandedness
(like the UCSC Genome Browser uses)
"dashed" a horizontal dashed line.
"crossed" a straight horizontal connector with an "X" on it
(Can be used when segments are not yet validated
by some internal experiments...)
The -connector_color option controls the color of the connector, if any.
The label is printed above the glyph. You may pass an anonymous subroutine to -label, in which case the
subroutine will be invoked with the feature as its single argument and is expected to return the string
to use as the label. If you provide the numeric value "1" to -label, the label will be read off the
feature's seqname(), info() and primary_tag() methods will be called until a suitable name is found. To
create a label with the text "1", pass the string "1 ". (A 1 followed by a space).
The description is printed below the glyph. You may pass an anonymous subroutine to -description, in
which case the subroutine will be invoked with the feature as its single argument and is expected to
return the string to use as the description. If you provide the numeric value "1" to -description, the
description will be read off the feature's source_tag() method. To create a description with the text
"1", pass the string "1 ". (A 1 followed by a space).
In the case of ACEDB Ace::Sequence feature objects, the feature's info(), Brief_identification() and
Locus() methods will be called to create a suitable description.
The -strand_arrow option, if true, requests that the glyph indicate which strand it is on, usually by
drawing an arrowhead. Not all glyphs will respond to this request. For historical reasons, -stranded is
a synonym for this option. Multisegmented features will draw an arrowhead on each component unless you
specify a value of "ends" to -strand_arrow, in which case only the rightmost component (for + strand
features) or the leftmost component (for - strand features) will have arrowheads.
sort_order: By default, features are drawn with a layout based only on the position of the feature,
assuring a maximal "packing" of the glyphs when bumped. In some cases, however, it makes sense to
display the glyphs sorted by score or some other comparison, e.g. such that more "important" features are
nearer the top of the display, stacked above less important features. The -sort_order option allows a
few different built-in values for changing the default sort order (which is by "left" position):
"low_score" (or "high_score") will cause features to be sorted from lowest to highest score (or vice
versa). "left" (or "default") and "right" values will cause features to be sorted by their position in
the sequence. "longest" (or "shortest") will cause the longest (or shortest) features to be sorted
first, and "strand" will cause the features to be sorted by strand: "+1" (forward) then "0" (unknown, or
NA) then "-1" (reverse). Finally, "name" will sort by the display_name of the features.
In all cases, the "left" position will be used to break any ties. To break ties using another field,
options may be strung together using a "|" character; e.g. "strand|low_score|right" would cause the
features to be sorted first by strand, then score (lowest to highest), then by "right" position in the
sequence.
Finally, a subroutine coderef with a $$ prototype can be provided. It will receive two glyph as
arguments and should return -1, 0 or 1 (see Perl's sort() function for more information). For example,
to sort a set of database search hits by bits (stored in the features' "score" fields), scaled by the log
of the alignment length (with "start" position breaking any ties):
sort_order = sub ($$) {
my ($glyph1,$glyph2) = @_;
my $a = $glyph1->feature;
my $b = $glyph2->feature;
( $b->score/log($b->length)
<=>
$a->score/log($a->length) )
||
( $a->start <=> $b->start )
}
It is important to remember to use the $$ prototype as shown in the example. Otherwise Bio::Graphics
will quit with an exception. The arguments are subclasses of Bio::Graphics::Glyph, not the features
themselves. While glyphs implement some, but not all, of the feature methods, to be safe call the two
glyphs' feature() methods in order to convert them into the actual features.
The '-always_sort' option, if true, will sort features even if bumping is turned off. This is useful if
you would like overlapping features to stack in a particular order. Features towards the end of the list
will overlay those towards the beginning of the sort order.
The -hilite option draws a colored box behind each feature using the indicated color. Typically you will
pass it a code ref that returns a color name. For example:
-hilite => sub { my $name = shift->display_name;
return 'yellow' if $name =~ /XYZ/ }
The -no_subparts option will prevent the glyph from searching its feature for subfeatures. This may
enhance performance if you know in advance that none of your features contain subfeatures.
SUBCLASSING Bio::Graphics::Glyph
By convention, subclasses are all lower-case. Begin each subclass with a preamble like this one:
package Bio::Graphics::Glyph::crossbox;
use strict;
use base qw(Bio::Graphics::Glyph);
Then override the methods you need to. Typically, just the draw() method will need to be overridden.
However, if you need additional room in the glyph, you may override calculate_height(), calculate_left()
and calculate_right(). Do not directly override height(), left() and right(), as their purpose is to
cache the values returned by their calculating cousins in order to avoid time-consuming recalculation.
A simple draw() method looks like this:
sub draw {
my $self = shift;
$self->SUPER::draw(@_);
my $gd = shift;
# and draw a cross through the box
my ($x1,$y1,$x2,$y2) = $self->calculate_boundaries(@_);
my $fg = $self->fgcolor;
$gd->line($x1,$y1,$x2,$y2,$fg);
$gd->line($x1,$y2,$x2,$y1,$fg);
}
This subclass draws a simple box with two lines criss-crossed through it. We first call our inherited
draw() method to generate the filled box and label. We then call calculate_boundaries() to return the
coordinates of the glyph, disregarding any extra space taken by labels. We call fgcolor() to return the
desired foreground color, and then call $gd->line() twice to generate the criss-cross.
For more complex draw() methods, see Bio::Graphics::Glyph::transcript and Bio::Graphics::Glyph::segments.
Please avoid using a specific image class (via "use GD" for example) within your glyph package. Instead,
rely on the image package passed to the draw() method. This approach allows for future expansion of
supported image classes without requiring glyph redesign. If you need access to the specific image
classes such as Polygon, Image, or Font, generate them like such:
sub draw {
my $self = shift;
my $image_class = shift;
my $polygon_package = $self->polygon_package->new()
...
}
BUGS
Please report them.
SEE ALSO
Bio::DB::GFF::Feature, Ace::Sequence, Bio::Graphics::Panel, Bio::Graphics::Track,
Bio::Graphics::Glyph::Factory, Bio::Graphics::Glyph::alignment, Bio::Graphics::Glyph::anchored_arrow,
Bio::Graphics::Glyph::arrow, Bio::Graphics::Glyph::box, Bio::Graphics::Glyph::broken_line,
Bio::Graphics::Glyph::cds, Bio::Graphics::Glyph::christmas_arrow, Bio::Graphics::Glyph::crossbox,
Bio::Graphics::Glyph::dashed_line, Bio::Graphics::Glyph::diamond, Bio::Graphics::Glyph::dna,
Bio::Graphics::Glyph::dot, Bio::Graphics::Glyph::dumbbell, Bio::Graphics::Glyph::ellipse,
Bio::Graphics::Glyph::ex, Bio::Graphics::Glyph::extending_arrow, Bio::Graphics::Glyph::flag,
Bio::Graphics::Glyph::gene, Bio::Graphics::Glyph::generic, Bio::Graphics::Glyph::graded_segments,
Bio::Graphics::Glyph::group, Bio::Graphics::Glyph::heterogeneous_segments, Bio::Graphics::Glyph::image,
Bio::Graphics::Glyph::lightning, Bio::Graphics::Glyph::line, Bio::Graphics::Glyph::merge_parts,
Bio::Graphics::Glyph::merged_alignment, Bio::Graphics::Glyph::minmax, Bio::Graphics::Glyph::oval,
Bio::Graphics::Glyph::pentagram, Bio::Graphics::Glyph::pinsertion, Bio::Graphics::Glyph::primers,
Bio::Graphics::Glyph::processed_transcript, Bio::Graphics::Glyph::protein,
Bio::Graphics::Glyph::ragged_ends, Bio::Graphics::Glyph::redgreen_box,
Bio::Graphics::Glyph::redgreen_segment, Bio::Graphics::Glyph::repeating_shape,
Bio::Graphics::Glyph::rndrect, Bio::Graphics::Glyph::ruler_arrow, Bio::Graphics::Glyph::saw_teeth,
Bio::Graphics::Glyph::segmented_keyglyph, Bio::Graphics::Glyph::segments,
Bio::Graphics::Glyph::so_transcript, Bio::Graphics::Glyph::span, Bio::Graphics::Glyph::splice_site,
Bio::Graphics::Glyph::stackedplot, Bio::Graphics::Glyph::ternary_plot, Bio::Graphics::Glyph::text_in_box,
Bio::Graphics::Glyph::three_letters, Bio::Graphics::Glyph::tic_tac_toe, Bio::Graphics::Glyph::toomany,
Bio::Graphics::Glyph::track, Bio::Graphics::Glyph::transcript, Bio::Graphics::Glyph::transcript2,
Bio::Graphics::Glyph::translation, Bio::Graphics::Glyph::triangle, Bio::Graphics::Glyph::two_bolts,
Bio::Graphics::Glyph::wave, Bio::Graphics::Glyph::weighted_arrow, Bio::Graphics::Glyph::whiskerplot,
Bio::Graphics::Glyph::xyplot
AUTHOR
Lincoln Stein <lstein@cshl.org>
Copyright (c) 2001 Cold Spring Harbor Laboratory
This library is free software; you can redistribute it and/or modify it under the same terms as Perl
itself. See DISCLAIMER.txt for disclaimers of warranty.