NAME

       nast-ier - NAST-based DNA alignment tool

DESCRIPTION

       The NAST-iEr alignment utility aligns a single raw nucleotide sequence against one or more NAST formatted
       sequences.

       The alignment algorithm involves global dynamic programming profile alignment to  fixed  (NAST-formatted)
       multiply aligned template sequences without any end-gap penalty.

       NAST-iEr is part of the microbiomeutil suite.

OPTIONS

   Required:
       --query_FASTA
              query database in FASTA format (seqs to NAST-align)

   Optional: (unless defaults are not set!)
       --db_NAST
              reference            database            in            NAST            format            (default:
              /usr/share/microbiomeutil-data/RESOURCES/rRNA16S.gold.NAST_ALIGNED.fasta)

       --db_FASTA
              reference           database            in            FASTA            format            (default:
              /usr/share/microbiomeutil-data/RESOURCES/rRNA16S.gold.fasta)

       --num_top_hits
              number of top hits to use for profile-alignment (default: 10)

       --Evalue
              Evalue cutoff for top hits (default: 1e-50)

SEE ALSO

       http://microbiomeutil.sourceforge.net/#A_NASTiEr

AUTHOR

       This  manual  page  was  written by Andreas Tille <tille@debian.org> but can be freely used for any other
       distribution.