NAME

       samtools fqidx - Indexes or queries regions from a fastq file

SYNOPSIS

       samtools fqidx ref.fastq [region1 [...]]

DESCRIPTION

       Index reference sequence in the FASTQ format or extract subsequence from indexed reference
       sequence. If no region is specified, fqidx will index the file and create  <ref.fastq>.fai
       on  the  disk. If regions are specified, the subsequences will be retrieved and printed to
       stdout in the FASTQ format.

       The input file can be compressed in the BGZF format.

       The sequences in the input file should all have different names.  If they do not, indexing
       will emit a warning about duplicate sequences and retrieval will only produce subsequences
       from the first sequence with the duplicated name.

       samtools fqidx should only be used on fastq files with a small number of entries.   Trying
       to  use  it  on a file containing millions of short sequencing reads will produce an index
       that is almost as big as the original file, and searches using the index will be very slow
       and use a lot of memory.

OPTIONS

       -o, --output FILE
               Write FASTQ to file rather than to stdout.

       -n, --length INT
               Length of FASTQ sequence line.  [60]

       -c, --continue
               Continue working if a non-existent region is requested.

       -r, --region-file FILE
               Read regions from a file. Format is chr:from-to, one per line.

       -i, --reverse-complement
               Output  the  sequence  as the reverse complement.  When this option is used, “/rc”
               will be appended to the sequence names.  To turn this off  or  change  the  string
               appended, use the --mark-strand option.

       --mark-strand TYPE
               Append strand indicator to sequence name.  TYPE can be one of:

               rc     Append '/rc' when writing the reverse complement.  This is the default.

               no     Do not append anything.

               sign   Append  '(+)'  for  forward  strand  or '(-)' for reverse complement.  This
                      matches the output of “bedtools getfasta -s”.

               custom,<pos>,<neg>
                      Append string <pos> to names when writing the forward strand and <neg> when
                      writing  the  reverse  strand.   Spaces are preserved, so it is possible to
                      move the indicator into  the  comment  part  of  the  description  line  by
                      including a leading space in the strings <pos> and <neg>.

               -h, --help
                      Print help message and exit.

AUTHOR

       Written  by Heng Li, with modifications by Andrew Whitwham and Robert Davies, all from the
       Sanger Institute.

SEE ALSO

       samtools(1), samtools-faidx(1), samtools-fasta(1), samtools-fastq(1)

       Samtools website: <http://www.htslib.org/>