NAME

       wigeon - reimplementation of the Pintail 16S DNA anomaly detection utility

DESCRIPTION

       WigeoN  examines the sequence conservation between a query and a trusted reference sequence, both in NAST
       alignment format. Based on the sequence identity between the query and the reference sequence,  there  is
       an  expected  amount  of variation among the alignment. If the observed variation is greater than the 95%
       quantile of the distribution of variation observed between non-anomalous sequences, then it is flagged as
       an anomaly.

       WigeoN is a flexible command-line based reimplementation of the Pintail algorithm Appl Environ Microbiol.
       2005 Dec;7112:7724-36.

       WigeoN is useful for flagging chimeras and anomalies only in near full-length 16S rRNA sequences.  WigeoN
       lacks sensitivity with sequences less than 1000 bp.

       To run WigeoN, you need NAST-formatted sequences generated by the nast-ier utility.

       WigeoN is  part of the microbiomeutil suite.

OPTIONS

   Required:
       --query_NAST
              multi-fasta file containing query sequences in alignment format

   Optional:
       --db_NAST
              db in NAST format

       --db_FASTA
              db in fasta format (megablast formatted)

       --num_top_hits
              default 1: uses only the single best match.

       --plot

       --DEBUG

       --exec_dir
              cd to exec_dir before running

SEE ALSO

       http://microbiomeutil.sourceforge.net/#A_WigeoN

AUTHOR

       This  manual  page  was  written by Andreas Tille <tille@debian.org> but can be freely used for any other
       distribution.