NAME

       filterBam - filter BAM file for use with AUGUSTUS tools

SYNOPSIS

       filterBam --in in.bam --out out.bam [options]

DESCRIPTION

       The input file must be sorted lexicographically by 'queryname', with e.g.

       ·   sort -k 1,1 [be aware: 'export LC_ALL=C' might be used  because sort ignores
           characters like ':'] Also, please bear in mind that this will require converting your
           BAM file into SAM.

       ·   samtools and bamtools provide facilities to do the sorting, but they are not
           guaranteed to work because of the problem mentioned above.

       ·   In the case of samtools, the command is: 'samtools sort [-n] file.bam'. The option
           [-n] should sort by query name, just as 'sort -k 10,10' would do in a PSL file.
           Without options, the sorting will be done by reference name and target coordinate,
           just as a  'sort -n -k 16,16 | sort -k 14,14' would do with PSL. For more information
           check the man page included in samtools distribution.

       ·   bamtools can also sort bam files: bamtools sort -queryname -in file.bam, but only
           provides the option to do it by queryname.

       If the option 'paired' is used, then alignment names must include suffixes /1,/2 or /f,/r.

OPTIONS

       --best
           output all best matches that satisfy minId and minCover (default 0)

       --noIntrons
           do not allow longer gaps -for RNA-RNA alignments- (default 0)

       --paired
           require that paired reads are on opposite strands of same target (default 0). NOTE:
           see prerequisite section above.

       --uniq
           take only best match, iff, second best is much worse  (default 0)

       --verbose
           output debugging info (default 0)

       --insertLimit n
           maximum assumed size of inserts (default 10)

       --maxIntronLen n
           maximal separation of paired reads (default 500000)

       --maxSortesTest n
           maximal sortedness (default 100000)

       --minCover n
           minimal percentage of coverage of the query read (default 80)

       --minId n
           minimal percentage of identity (default 92)

       --minIntronLen n
           minimal intron length  (default 35)

       --uniqThresh n
           threshold % for uniq, second best must be at most this fraction of best (default 0.96)

       --commonGeneFile s
           file name in which to write cases where one read maps several different genes

       --pairBedFile s
           file name of pairedness coverage: a BED format file in which for each position the
           number of filtered read pairs is reported that contain the position in or between the
           reads

       --pairwiseAlignments
           use in case alignments were done in pairwise fashion (default:  0)

AUTHORS

       AUGUSTUS was written by M. Stanke, O. Keller, S. König, L. Gerischer and L. Romoth.

ADDITIONAL DOCUMENTATION

       An exhaustive documentation can be found in the file /usr/share/doc/augustus/README.md.gz.

                                                                                     FILTERBAM(1)