Provided by: pftools_3.2.12-1_amd64 
NAME
ptof - convert a protein profile into a frame-search profile
SYNOPSIS
ptof [ -hlr ] [ -B init/term_score ] [ -F frameshift ] [ -I insert_multiplier ] [ -X
stop_codon ] [ -Y intron_open ] [ -Z intron_extend ] [ protein_profile | - ] [
parameters ]
DESCRIPTION
ptof converts a protein profile (generated for instance by pftools programs pfmake(1),
gtop(1) or htop(1)) into a so-called "frame-search profile". A frame-search profile is
used to search an "interleaved frame-translated" DNA sequence (generated by pftools
program 2ft(1)) for occurrences of a protein sequence motif. An "interleaved frame-
translated" DNA sequence is an amino acid sequence corresponding to the N-2 overlapping
codons of a DNAsequence of length N. Note that in such a sequence, the character 'O' is
used to represent stop codons.
The conversion procedure works as follows: The protein profile is expanded in length by a
factor of three to accommodate three translated codons per original match position. Two
dummy match positions are placed between two consecutive significant match positions
imported from the original profile. The original insert positions are placed between
pairs of adjacent dummy match positions. The initiation, termination, and transition
scores of the original insert positions are left unchanged; the insert extension scores
are divided by a factor of 3, or by the value of the command-line option -I. The two
insert positions flanking the significant match positions serve to accommodate frame-shift
errors and introns, respectively. The frame-shift insert position allows free insertion
opening combined with a high insert extension penalty (command-line option -F) whereas the
intron insertion position has high opening but low extension penalties (command line
options -Y and -Z). The deletion opening and closing penalties next to the significant
match positions are set to values that ensure that the total cost of a single-base
deletion is the same as the cost of a single base-insertion at a frame-shift insert
position. Furthermore, the alphabet of the original profile is extended by the stop codon
symbol 'O' which is assigned a constant negative value (command-line option -X) at
significant match positions, and zero at dummy match positions. At insert positions, it is
set to the average of the other insert extension scores.
OPTIONS
protein_profile
Input protein profile.
The protein profile contained in this file will be converted into a frame-search
profile. If the filename is replaced by a '-', ptof will read the profile from
stdin.
-h Display usage help text.
-l Remove output line length limit. Individual lines of the output profile can exceed
a length of 132 characters, removing the need to wrap them over several lines.
-r Frame-search parameters are given in normalized score units. This option will only
be considered if a linear normalization function with priority over all other
normalization functions is specified in the profile. In this case, the frame-
search scores specified on the command line will be divided by the slope (R2
parameter) of the normalization function. This option is particularly useful for
profiles which are already scaled in units that can be interpreted as
−Log(P)-values, e.g. bits.
-B init/term_score
Minimal initiation and termination score.
All internal and external initiation and termination scores will be set to this
value if the corresponding value in the original profile is lower than this value.
This parameter is used to impose a more local alignment behavior on the frame-
search profile in order to deal with discontinuities in DNA sequences (long
introns, alternative splicing, chimeric clones, etc.)
Default: -50 (-0.5 with option -r)
-F frameshift
Frame-shift error penalty.
Default: -100 (-1.0 with option -r)
-I insert_multiplier
Insert score multiplier.
The values of the original insert extension scores will be multiplied by this
factor in order to compensate for the fact that a single amino acid corresponds to
three overlapping codon positions in the target sequence.
Default: 1/3
-X stop_codon
Stop codon penalty.
Default: -100 (-1.0 with option -r)
-Y intron_open
Intron opening penalty.
Default: -300 (-3.0 with option -r)
-Z intron_extend
Intron extension penalty.
Default: -1 (-0.01 with option -r)
PARAMETERS
Note: for backwards compatibility, release 2.3 of the pftools package will parse the
version 2.2 style parameters, but these are deprecated and the corresponding option
(refer to the options section) should be used instead.
B=# Minimal initiation and termination score.
Use option -B instead.
F=# Frame-shift error penalty.
Use option -F instead.
I=# Insert score multiplier.
Use option -I instead.
X=# Stop codon penalty.
Use option -X instead.
Y=# Intron opening penalty.
Use option -Y instead.
Z=# Intron extension penalty.
Use option -Z instead.
EXAMPLES
(1) ptof -r -F -1.2 -I 0.6 -X -1.5 -B -0.5 sh3.prf > sh3.fsp
2ft - < R76849.seq | pfsearch -fy -C 5.0 sh3.fsp -
The protein domain profile in 'sh3.prf' is first converted into a frame-search
profile 'sh3.fsp'. Then both strands of the Fasta-formatted EST sequence in
'R76849.seq' (GenBank/EMBL-accession: R76849) are converted into interleaved frame-
translated protein sequences and searched for SH3 domains with the frame-search
profile generated in the preceding step.
The output may be compared to the result of a more conventional search strategy
using a protein profile in conjunction with a six-frame translation of the same DNA
sequence:
6ft - < R76849.seq | pfsearch -fy -C 5.0 sh3.prf -
EXIT CODE
On successful completion of its task, ptof will return an exit code of 0. If an error
occurs, a diagnostic message will be output on standard error and the exit code will be
different from 0. When conflicting options where passed to the program but the task could
nevertheless be completed, warnings will be issued on standard error.
SEE ALSO
pfscan(1), pfsearch(1), 2ft(1), 6ft(1)
AUTHOR
The pftools package was developed by Philipp Bucher.
Any comments or suggestions should be addressed to <pftools@sib.swiss>.