Provided by: libbio-db-seqfeature-perl_1.7.4-2_all
NAME
Bio::DB::SeqFeature::Store::DBI::mysql -- Mysql implementation of Bio::DB::SeqFeature::Store
SYNOPSIS
use Bio::DB::SeqFeature::Store; # Open the sequence database my $db = Bio::DB::SeqFeature::Store->new(-adaptor => 'DBI::mysql', -dsn => 'dbi:mysql:test'); # get a feature from somewhere my $feature = Bio::SeqFeature::Generic->new(...); # store it $db->store($feature) or die "Couldn't store!"; # primary ID of the feature is changed to indicate its primary ID # in the database... my $id = $feature->primary_id; # get the feature back out my $f = $db->fetch($id); # change the feature and update it $f->start(100); $f->update($f) or die "Couldn't update!"; # searching... # ...by id my @features = $db->fetch_many(@list_of_ids); # ...by name @features = $db->get_features_by_name('ZK909'); # ...by alias @features = $db->get_features_by_alias('sma-3'); # ...by type @features = $db->get_features_by_name('gene'); # ...by location @features = $db->get_features_by_location(-seq_id=>'Chr1',-start=>4000,-end=>600000); # ...by attribute @features = $db->get_features_by_attribute({description => 'protein kinase'}) # ...by the GFF "Note" field @result_list = $db->search_notes('kinase'); # ...by arbitrary combinations of selectors @features = $db->features(-name => $name, -type => $types, -seq_id => $seqid, -start => $start, -end => $end, -attributes => $attributes); # ...using an iterator my $iterator = $db->get_seq_stream(-name => $name, -type => $types, -seq_id => $seqid, -start => $start, -end => $end, -attributes => $attributes); while (my $feature = $iterator->next_seq) { # do something with the feature } # ...limiting the search to a particular region my $segment = $db->segment('Chr1',5000=>6000); my @features = $segment->features(-type=>['mRNA','match']); # getting & storing sequence information # Warning: this returns a string, and not a PrimarySeq object $db->insert_sequence('Chr1','GATCCCCCGGGATTCCAAAA...'); my $sequence = $db->fetch_sequence('Chr1',5000=>6000); # what feature types are defined in the database? my @types = $db->types; # create a new feature in the database my $feature = $db->new_feature(-primary_tag => 'mRNA', -seq_id => 'chr3', -start => 10000, -end => 11000);
DESCRIPTION
Bio::DB::SeqFeature::Store::mysql is the Mysql adaptor for Bio::DB::SeqFeature::Store. You will not create it directly, but instead use Bio::DB::SeqFeature::Store->new() to do so. See Bio::DB::SeqFeature::Store for complete usage instructions. Using the Mysql adaptor Before you can use the adaptor, you must use the mysqladmin tool to create a database and establish a user account with write permission. In order to use "fast" loading, the user account must have "file" privileges. To establish a connection to the database, call Bio::DB::SeqFeature::Store->new(-adaptor=>'DBI::mysql',@more_args). The additional arguments are as follows: Argument name Description ------------- ----------- -dsn The database name. You can abbreviate "dbi:mysql:foo" as "foo" if you wish. -user Username for authentication. -pass Password for authentication. -namespace A prefix to attach to each table. This allows you to have several virtual databases in the same physical database. -temp Boolean flag. If true, a temporary database will be created and destroyed as soon as the Store object goes out of scope. (synonym -temporary) -autoindex Boolean flag. If true, features in the database will be reindexed every time they change. This is the default. -tmpdir Directory in which to place temporary files during "fast" loading. Defaults to File::Spec->tmpdir(). (synonyms -dump_dir, -dumpdir, -tmp) -dbi_options A hashref to pass to DBI->connect's 4th argument, the "attributes." (synonyms -options, -dbi_attr) -write Pass true to open database for writing or updating. If successful, a new instance of Bio::DB::SeqFeature::Store::DBI::mysql will be returned. In addition to the standard methods supported by all well-behaved Bio::DB::SeqFeature::Store databases, several following adaptor-specific methods are provided. These are described in the next sections. types Title : types Usage : @type_list = $db->types Function: Get all the types in the database Returns : array of Bio::DB::GFF::Typename objects Args : none Status : public toplevel_types Title : toplevel_types Usage : @type_list = $db->toplevel_types Function: Get the toplevel types in the database Returns : array of Bio::DB::GFF::Typename objects Args : none Status : public This is similar to types() but only returns the types of INDEXED (toplevel) features.