Provided by: libbio-perl-perl_1.7.8-1_all
NAME
Bio::Index::Qual - Interface for indexing (multiple) fasta qual files
SYNOPSIS
# Complete code for making an index for several # qual files use Bio::Index::Qual; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Qual->new( '-filename' => $Index_File_Name, '-write_flag' => 1); $inx->make_index(@ARGV); # Print out several sequences present in the index # in Qual format use Bio::Index::Qual; use strict; my $Index_File_Name = shift; my $inx = Bio::Index::Qual->new('-filename' => $Index_File_Name); my $out = Bio::SeqIO->new('-format' => 'qual','-fh' => \*STDOUT); foreach my $id (@ARGV) { my $seq = $inx->fetch($id); # Returns Bio::Seq object $out->write_seq($seq); } # or, alternatively my $id; my $seq = $inx->get_Seq_by_id($id); #identical to fetch
DESCRIPTION
Inherits functions for managing dbm files from Bio::Index::Abstract.pm, and provides the basic funtionality for indexing qual files, and retrieving the sequence from them. For best results 'use strict'. Bio::Index::Qual supports the Bio::DB::BioSeqI interface, meaning it can be used as a Sequence database for other parts of bioperl Additional example code is available in scripts/index. Note that by default the key for the sequence will be the first continuous string after the '>' in the qual header. If you want to use a specific substring of the qual header you must use the id_parser() method. You can also set or customize the unique key used to retrieve by writing your own function and calling the id_parser() method. For example: $inx->id_parser(\&get_id); # make the index $inx->make_index($file_name); # here is where the retrieval key is specified sub get_id { my $line = shift; $line =~ /^(\d+)/; $1; }
FEED_BACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://github.com/bioperl/bioperl-live/issues
AUTHOR - James Gilbert, Mark Johnson
Email - jgrg@sanger.ac.uk, johnsonm-at-gmail-dot-com
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ _file_format Title : _file_format Function: The file format for this package, which is needed by the SeqIO system when reading the sequence. Returns : 'qual' _index_file Title : _index_file Usage : $index->_index_file( $file_name, $i ) Function: Specialist function to index QUAL format files. Is provided with a filename and an integer by make_index in its SUPER class. Example : Returns : Args : id_parser Title : id_parser Usage : $index->id_parser( CODE ) Function: Stores or returns the code used by record_id to parse the ID for record from a string. Useful for (for instance) specifying a different parser for different flavours of Qual file. Returns \&default_id_parser (see below) if not set. If you supply your own id_parser subroutine, then it should expect a qual description line. An entry will be added to the index for each string in the list returned. Example : $index->id_parser( \&my_id_parser ) Returns : ref to CODE if called without arguments Args : CODE default_id_parser Title : default_id_parser Usage : $id = default_id_parser( $header ) Function: The default Qual ID parser for Qual.pm Returns $1 from applying the regexp /^>\s*(\S+)/ to $header. Returns : ID string Args : a qual header line string