Provided by: libbio-perl-perl_1.7.8-1_all
NAME
Bio::Tools::GuessSeqFormat - Module for determining the sequence format of the contents of a file, a string, or through a filehandle.
SYNOPSIS
# To guess the format of a flat file, given a filename: my $guesser = Bio::Tools::GuessSeqFormat->new( -file => $filename ); my $format = $guesser->guess; # To guess the format from an already open filehandle: my $guesser = Bio::Tools::GuessSeqFormat->new( -fh => $filehandle ); my $format = $guesser->guess; # The filehandle will be returned to its original position. Note that this # filehandle can be STDIN. # To guess the format of one or several lines of text (with # embedded newlines): my $guesser = Bio::Tools::GuessSeqFormat->new( -text => $linesoftext ); my $format = $guesser->guess; # To create a Bio::Tools::GuessSeqFormat object and set the # filename, filehandle, or line to parse afterwards: my $guesser = Bio::Tools::GuessSeqFormat->new(); $guesser->file($filename); $guesser->fh($filehandle); $guesser->text($linesoftext); # To guess in one go, given e.g. a filename: my $format = Bio::Tools::GuessSeqFormat->new( -file => $filename )->guess;
DESCRIPTION
Bio::Tools::GuessSeqFormat tries to guess the format ("swiss", "pir", "fasta" etc.) of the sequence or MSA in a file, in a scalar, or through a filehandle. The guess() method of a Bio::Tools::GuessSeqFormat object will examine the data, line by line, until it finds a line to which only one format can be assigned. If no conclusive guess can be made, undef is returned. If the Bio::Tools::GuessSeqFormat object is given a filehandle, e.g. STDIN, it will be restored to its original position on return from the guess() method. Formats Tests are currently implemented for the following formats: • ACeDB ("ace") • Blast ("blast") • ClustalW ("clustalw") • Codata ("codata") • EMBL ("embl") • FastA sequence ("fasta") • FastQ sequence ("fastq") • FastXY/FastA alignment ("fastxy") • Game XML ("game") • GCG ("gcg") • GCG Blast ("gcgblast") • GCG FastA ("gcgfasta") • GDE ("gde") • Genbank ("genbank") • Genscan ("genscan") • GFF ("gff") • HMMER ("hmmer") • PAUP/NEXUS ("nexus") • Phrap assembly file ("phrap") • NBRF/PIR ("pir") • Mase ("mase") • Mega ("mega") • GCG/MSF ("msf") • Pfam ("pfam") • Phylip ("phylip") • Prodom ("prodom") • Raw ("raw") • RSF ("rsf") • Selex ("selex") • Stockholm ("stockholm") • Swissprot ("swiss") • Tab ("tab") • Variant Call Format ("vcf")
FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: https://github.com/bioperl/bioperl-live/issues
AUTHOR
Andreas Kähäri, andreas.kahari@ebi.ac.uk
CONTRIBUTORS
Heikki Lehväslaiho, heikki-at-bioperl-dot-org Mark A. Jensen, maj-at-fortinbras-dot-us
METHODS
Methods available to Bio::Tools::GuessSeqFormat objects are described below. Methods with names beginning with an underscore are considered to be internal. new Title : new Usage : $guesser = Bio::Tools::GuessSeqFormat->new( ... ); Function : Creates a new object. Example : See SYNOPSIS. Returns : A new object. Arguments : -file The filename of the file whose format is to be guessed, e.g. STDIN, or -fh An already opened filehandle from which a text stream may be read, or -text A scalar containing one or several lines of text with embedded newlines. If more than one of the above arguments are given, they are tested in the order -text, -file, -fh, and the first available argument will be used. file Title : file Usage : $guesser->file($filename); $filename = $guesser->file; Function : Gets or sets the current filename associated with an object. Returns : The new filename. Arguments : The filename of the file whose format is to be guessed. A call to this method will clear the current filehandle and the current lines of text associated with the object. fh Title : fh Usage : $guesser->fh($filehandle); $filehandle = $guesser->fh; Function : Gets or sets the current filehandle associated with an object. Returns : The new filehandle. Arguments : An already opened filehandle from which a text stream may be read. A call to this method will clear the current filename and the current lines of text associated with the object. text Title : text Usage : $guesser->text($linesoftext); $linesofext = $guesser->text; Function : Gets or sets the current text associated with an object. Returns : The new lines of texts. Arguments : A scalar containing one or several lines of text, including embedded newlines. A call to this method will clear the current filename and the current filehandle associated with the object. guess Title : guess Usage : $format = $guesser->guess; @format = $guesser->guess; # if given a line of text Function : Guesses the format of the data accociated with the object. Returns : A format string such as "swiss" or "pir". If a format can not be found, undef is returned. Arguments : None. If the object is associated with a filehandle, the position of the filehandle will be returned to its original position before the method returns.
HELPER SUBROUTINES
All helper subroutines will, given a line of text and the line number of the same line, return 1 if the line possibly is from a file of the type that they perform a test of. A zero return value does not mean that the line is not part of a certain type of file, just that the test did not find any characteristics of that type of file in the line. _possibly_ace From bioperl test data, and from "http://www.isrec.isb-sib.ch/DEA/module8/B_Stevenson/Practicals/transcriptome_recon/transcriptome_recon.html". _possibly_blast From various blast results. _possibly_bowtie Contributed by kortsch. _possibly_clustalw From "http://www.ebi.ac.uk/help/formats.html". _possibly_codata From "http://www.ebi.ac.uk/help/formats.html". _possibly_embl From "http://www.ebi.ac.uk/embl/Documentation/User_manual/usrman.html#3.3". _possibly_fasta From "http://www.ebi.ac.uk/help/formats.html". _possibly_fastq From bioperl test data. _possibly_fastxy From bioperl test data. _possibly_game From bioperl testdata. _possibly_gcg From bioperl, Bio::SeqIO::gcg. _possibly_gcgblast From bioperl testdata. _possibly_gcgfasta From bioperl testdata. _possibly_gde From "http://www.ebi.ac.uk/help/formats.html". _possibly_genbank From "http://www.ebi.ac.uk/help/formats.html". Format of [apparantly optional] file header from "http://www.umdnj.edu/rcompweb/PA/Notes/GenbankFF.htm". (TODO: dead link) _possibly_genscan From bioperl test data. _possibly_gff From bioperl test data. _possibly_hmmer From bioperl test data. _possibly_nexus From "http://paup.csit.fsu.edu/nfiles.html". _possibly_mase From bioperl test data. More detail from "http://www.umdnj.edu/rcompweb/PA/Notes/GenbankFF.htm" (TODO: dead link) _possibly_mega From the ensembl broswer (AlignView data export). _possibly_msf From "http://www.ebi.ac.uk/help/formats.html". _possibly_phrap From "http://biodata.ccgb.umn.edu/docs/contigimage.html". (TODO: dead link) From "http://genetics.gene.cwru.edu/gene508/Lec6.htm". (TODO: dead link) From bioperl test data ("*.ace.1" files). _possibly_pir From "http://www.ebi.ac.uk/help/formats.html". The ".,()" spotted in bioperl test data. _possibly_pfam From bioperl test data. _possibly_phylip From "http://www.ebi.ac.uk/help/formats.html". Initial space allowed on first line (spotted in ensembl AlignView exported data). _possibly_prodom From "http://prodom.prabi.fr/prodom/current/documentation/data.php". _possibly_raw From "http://www.ebi.ac.uk/help/formats.html". _possibly_rsf From "http://www.ebi.ac.uk/help/formats.html". _possibly_selex From "http://www.ebc.ee/WWW/hmmer2-html/node27.html". Assuming presence of Selex file header. Data exported by Bioperl on Pfam and Selex formats are identical, but Pfam file only holds one alignment. _possibly_stockholm From bioperl test data. _possibly_swiss From "http://ca.expasy.org/sprot/userman.html#entrystruc". _possibly_tab Contributed by Heikki. _possibly_vcf From "http://www.1000genomes.org/wiki/analysis/vcf4.0". Assumptions made about sanity - format and date lines are line 1 and 2 respectively. This is not specified in the format document.