Provided by: bio-tradis_1.4.5+dfsg2-1_all
NAME
Bio::Tradis::RemoveTags - Remove tags from seqs a fastq file
VERSION
version 1.4.5
SYNOPSIS
Reads in a fastq file with tradis tags already attached to the start of the sequence Removes tags from the sequence and quality strings Outputs a file *.rmtag.fastq unless an out file is specified use Bio::Tradis::RemoveTags; my $pipeline = Bio::Tradis::RemoveTags->new(fastqfile => 'abc', tag => 'abc'); $pipeline->remove_tags();
PARAMETERS
Required • "fastqfile" - path to/name of file to filter • "tag" - TraDIS tag to remove Optional • "mismatch" - number of mismatches to allow when removing the tag. Default = 0 • "outfile" - defaults to "file.rmtag.fastq" for and input file named "file.fastq"
METHODS
"remove_tags" - output all reads with the tags removed to "outfile"
AUTHOR
Carla Cummins <path-help@sanger.ac.uk>
COPYRIGHT AND LICENSE
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. This is free software, licensed under: The GNU General Public License, Version 3, June 2007