NAME

       primer_designer.pl -- command-line interface Bio::PrimerDesigner

SYNOPSIS

           ./primer_designer.pl [options] [dna_sequence or file]

         Options:

         -p|--program   Program (default "primer3")
         -n|--number    Number of primer sets to return (default "5")
         -b|--binary    Path to binary executable (default "/usr/local/bin")
         -m|--method    "local" or "remote" (default "local")
         -u|--url       URL of remote primer3/e-PCR system (default
                          "aceserver.biotech.ubc.ca/cgi-bin/primer_designer.cgi")
         --list-aliases Print alias list to primer3 input options and exit
         --list-params  Print a list of primer3/e-PCR input options and exit
         -h|--help      Print help and exit

DESCRIPTION

       This script tests/demonstrates the Bio::PrimerDesigner interface to primer3 and e-PCR.  It
       can be used with a local installation of the unix binaries or remotely via an HTTP/CGI
       request.

AUTHOR

       Copyright (C) 2003-2009 Sheldon McKay <mckays@cshl.edu>, Ken Youens-Clark
       <kclark@cpan.org>.

SEE ALSO

       Bio::PrimerDesigner.

LICENSE

       This program is free software; you can redistribute it and/or modify it under the terms of
       the GNU General Public License as published by the Free Software Foundation; version 3 or
       any later version.

       This program is distributed in the hope that it will be useful, but WITHOUT ANY WARRANTY;
       without even the implied warranty of MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE.
       See the GNU General Public License for more details.

       You should have received a copy of the GNU General Public License along with this program;
       if not, write to the Free Software Foundation, Inc., 51 Franklin Street, Fifth Floor,
       Boston, MA 02110-1301 USA.