Provided by: velvet-long_1.2.10+dfsg1-8_amd64
NAME
velveth_127_long - simple hashing program (MAXKMERLENGTH = 127, LONGSEQUENCES)
DESCRIPTION
velveth - simple hashing program Version 1.2.09
OPTIONS
-strand_specific : for strand specific transcriptome sequencing data (default: off) -reuse_Sequences : reuse Sequences file (or link) already in directory (no need to provide original filenames in this case (default: off) -reuse_binary : reuse binary sequences file (or link) already in directory (no need to provide original filenames in this case (default: off) -noHash : simply prepare Sequences file, do not hash reads or prepare Roadmaps file (default: off) -create_binary : create binary CnyUnifiedSeq file (default: off) Synopsis: - Short single end reads: velveth Assem 29 -short -fastq s_1_sequence.txt - Paired-end short reads (remember to interleave paired reads): velveth Assem 31 -shortPaired -fasta interleaved.fna - Paired-end short reads (using separate files for the paired reads) velveth Assem 31 -shortPaired -fasta -separate left.fa right.fa - Two channels and some long reads: velveth Assem 43 -short -fastq unmapped.fna -longPaired -fasta SangerReads.fasta - Three channels: velveth Assem 35 -shortPaired -fasta pe_lib1.fasta -shortPaired2 pe_lib2.fasta -short3 se_lib1.fa Output: directory/Roadmaps directory/Sequences [Both files are picked up by graph, so please leave them there]
COPYRIGHT
Copyright 2007, 2008 Daniel Zerbino (zerbino@ebi.ac.uk) This is free software; see the source for copying conditions. There is NO warranty; not even for MERCHANTABILITY or FITNESS FOR A PARTICULAR PURPOSE. Compilation settings: CATEGORIES = 2 MAXKMERLENGTH = 127 OPENMP LONGSEQUENCES Usage: ./velveth directory hash_length {[-file_format][-read_type][-separate|-interleaved] filename1 [filename2 ...]} {...} [options] directory : directory name for output files hash_length : EITHER an odd integer (if even, it will be decremented) <= 127 (if above, will be reduced) : OR: m,M,s where m and M are odd integers (if not, they will be decremented) with m < M <= 127 (if above, will be reduced) and s is a step (even number). Velvet will then hash from k=m to k=M with a step of s filename : path to sequence file or - for standard input File format options: -fasta -fastq -raw -fasta.gz -fastq.gz -raw.gz -sam -bam -fmtAuto (Note: -fmtAuto will detect fasta or fastq, and will try the following programs for decompression : gunzip, pbunzip2, bunzip2 File layout options for paired reads (only for fasta and fastq formats): -interleaved : File contains paired reads interleaved in the one file (default) -separate : Read 2 separate files for paired reads Read type options: -short -shortPaired -short2 -shortPaired2 -long -longPaired -reference