Provided by: bio-tradis_1.4.5+dfsg2-2_all
NAME
Bio::Tradis::Parser::Bam - Very basic BAM parser. Limited functionality.
VERSION
version 1.4.5
SYNOPSIS
Parses BAM files and gives access to basic info in them. use Bio::Tradis::Parser::Bam; my $pipeline = Bio::Tradis::Parser::Bam->new(file => 'abc'); $pipeline->read_info; $pipeline->next_read; $pipeline->seq_info; $pipeline->is_mapped; $pipeline->is_reverse; =seq_info Reads BAM header and returns a hash (keys are sequence ids, values are hash refs with keys as tags (like LN and M5)) =next_read Moves _currentread to the next entry in the BAM. Returns 0 if EOF. =read_info Returns info from _currentread = hash reference with field name as key. Standard fields are named as per the SAM format specification: 1 : QNAME 2 : FLAG 3 : RNAME 4 : POS 5 : MAPQ 6 : CIGAR 7 : RNEXT 8 : PNEXT 9 : TLEN 10 : SEQ 11 : QUAL Additional fields will use their tag names. Complete line is returned with key READ =is_mapped Parses the flag for the current read and determines if mapped. Returns 0 or 1. =is_reverse Parses the flag for the current read and determines if reverse complemented. Returns 0 or 1.
AUTHOR
Carla Cummins <path-help@sanger.ac.uk>
COPYRIGHT AND LICENSE
This software is Copyright (c) 2013 by Wellcome Trust Sanger Institute. This is free software, licensed under: The GNU General Public License, Version 3, June 2007