NAME

       Barcode_split - simple tool to split sequencing summary report in per barcodes

DESCRIPTION

       usage: Barcode_split [-h] [--version] --summary_file [SUMMARY_FILE ...]

       [--barcode_file [BARCODE_FILE ...]]
              [--output_dir     OUTPUT_DIR]     [--output_unclassified]    [--min_barcode_percent
              MIN_BARCODE_PERCENT] [-v | -q]

       Barcode_split is a simple tool to split sequencing summary report in per barcodes

   optional arguments:
       -h, --help
              show this help message and exit

       --version
              show program's version number and exit

       --summary_file [SUMMARY_FILE ...], -f [SUMMARY_FILE ...]
              Path    to    a    sequencing_summary    generated    by    Albacore    1.0.0     +
              (read_fast5_basecaller.py)  /  Guppy  2.1.3+  (guppy_basecaller). One can also pass
              multiple space separated file paths or a UNIX style regex matching multiple files

       --barcode_file [BARCODE_FILE ...], -b [BARCODE_FILE ...]
              Path to the barcode_file generated by Guppy 2.1.3+ (guppy_barcoder)  or  Deepbinner
              0.2.0+. One can also pass multiple space separated file paths or a UNIX style regex
              matching multiple files

       --output_dir OUTPUT_DIR, -o OUTPUT_DIR
              Folder where to output split barcode data (default: current dir

       --output_unclassified, -u
              If given, unclassified barcodes are also written in a file.  By  default  they  are
              skiped

       --min_barcode_percent MIN_BARCODE_PERCENT, -p MIN_BARCODE_PERCENT
              Minimal percent of total reads to retain barcode label. If below, the barcode value
              is set as `unclassified` (default: 0.1)

       -v, --verbose
              Increase verbosity

       -q, --quiet
              Reduce verbosity

AUTHOR

        This manpage was written by Nilesh Patra for the Debian distribution and
        can be used for any other usage of the program.