NAME

       mac3_randsample - Model-based Analysis for ChIP-Sequencing

DESCRIPTION

       usage: macs3 randsample [-h] -i IFILE [IFILE ...] (-p PERCENTAGE | -n NUMBER)

       [--seed SEED] [-o OUTPUTFILE] [--outdir OUTDIR]
              [-s  TSIZE] [-f {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}]
              [--buffer-size BUFFER_SIZE] [--verbose VERBOSE]

   options:
       -h, --help
              show this help message and exit

       -i IFILE [IFILE ...], --ifile IFILE [IFILE ...]
              Alignment file. If multiple files are given as '-t A B C', then they  will  all  be
              read and combined. REQUIRED.

       -p PERCENTAGE, --percentage PERCENTAGE
              Percentage  of tags you want to keep. Input 80.0 for 80%. This option can't be used
              at the same time with -n/--num. If the setting is 100, it will keep all  the  reads
              and  convert  any  format that MACS3 supports into BED or BEDPE (if input is BAMPE)
              format. REQUIRED

       -n NUMBER, --number NUMBER
              Number of tags you want to keep. Input 8000000 or 8e+6 for 8 million.  This  option
              can't  be  used at the same time with -p/--percent. Note that the number of tags in
              output is approximate as the number specified here.  REQUIRED

       --seed SEED
              Set the random seed while down sampling data. Must be  a  non-negative  integer  in
              order  to  be  effective.  If  you want more reproducible results, please specify a
              random seed and record it.DEFAULT: not set

       -o OUTPUTFILE, --ofile OUTPUTFILE
              Output BED file name. If not specified, will write to standard output. Note, if the
              input format is BAMPE or BEDPE, the output will be in BEDPE format. DEFAULT: stdout

       --outdir OUTDIR
              If  specified  all  output  files  will  be written to that directory. Default: the
              current working directory

       -s TSIZE, --tsize TSIZE
              Tag size. This will override the auto detected tag size. DEFAULT: Not set

       -f      {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE},       --format
       {AUTO,BAM,SAM,BED,ELAND,ELANDMULTI,ELANDEXPORT,BOWTIE,BAMPE,BEDPE}
              Format  of  tag  file, "AUTO", "BED" or "ELAND" or "ELANDMULTI" or "ELANDEXPORT" or
              "SAM" or "BAM" or "BOWTIE" or "BAMPE" or "BEDPE".  The  default  AUTO  option  will
              macs3  randsample  decide  which format the file is. Please check the definition in
              README  file   if   you   choose   ELAND/ELANDMULTI/ELANDEXPORT/SAM/BAM/BOWTIE   or
              BAMPE/BEDPE. DEFAULT: "AUTO"

       --buffer-size BUFFER_SIZE
              Buffer  size  for  incrementally  increasing  internal  array  size  to store reads
              alignment information. In most cases, you don't  have  to  change  this  parameter.
              However,  if  there  are  large  number  of  chromosomes/contigs/scaffolds  in your
              alignment, it's recommended to specify a smaller buffer size in order  to  decrease
              memory usage (but it will take longer time to read alignment files). Minimum memory
              requested for reading an alignment file is about # of CHROMOSOME * BUFFER_SIZE *  8
              Bytes. DEFAULT: 100000

       --verbose VERBOSE
              Set  verbose  level.  0:  only  show  critical  message, 1: show additional warning
              message, 2: show process information, 3: show debug messages. If you want  to  know
              where are the duplicate reads, use 3. DEFAULT:2