NAME

       bp_load_gff.pl - Load a Bio::DB::GFF database from GFF files.

SYNOPSIS

         % bp_load_gff.pl -d testdb -u user -p pw
            --dsn 'dbi:mysql:database=dmel_r5_1;host=myhost;port=myport'
               dna1.fa dna2.fa features1.gff features2.gff ...

DESCRIPTION

       This script loads a Bio::DB::GFF database with the features contained in a list of GFF
       files and/or FASTA sequence files.  You must use the exact variant of GFF described in
       Bio::DB::GFF.  Various command-line options allow you to control which database to load
       and whether to allow an existing database to be overwritten.

       This script uses the Bio::DB::GFF interface, and so works with all database adaptors
       currently supported by that module (MySQL, Oracle, PostgreSQL soon).  However, it is slow.
       For faster loading, see the MySQL-specific bp_bulk_load_gff.pl and bp_fast_load_gff.pl
       scripts.

   NOTES
       If the filename is given as "-" then the input is taken from standard input. Compressed
       files (.gz, .Z, .bz2) are automatically uncompressed.

       FASTA format files are distinguished from GFF files by their filename extensions.  Files
       ending in .fa, .fasta, .fast, .seq, .dna and their uppercase variants are treated as FASTA
       files.  Everything else is treated as a GFF file.  If you wish to load -fasta files from
       STDIN, then use the -f command-line swith with an argument of '-', as in

           gunzip my_data.fa.gz | bp_fast_load_gff.pl -d test -f -

       On the first load of a database, you will see a number of "unknown table" errors.  This is
       normal.

       About maxfeature: the default value is 100,000,000 bases.  If you have features that are
       close to or greater that 100Mb in length, then the value of maxfeature should be increased
       to 1,000,000,000, or another power of 10.

COMMAND-LINE OPTIONS

       Command-line options can be abbreviated to single-letter options.  e.g. -d instead of
       --database.

          --dsn     <dsn>       Data source (default dbi:mysql:test)
          --adaptor <adaptor>   Schema adaptor (default dbi::mysqlopt)
          --user    <user>      Username for mysql authentication
          --pass    <password>  Password for mysql authentication
          --fasta   <path>      Fasta file or directory containing fasta files for the DNA
          --create              Force creation and initialization of database
          --maxfeature          Set the value of the maximum feature size (default 100 Mb; must be a power of 10)
          --group               A list of one or more tag names (comma or space separated)
                                 to be used for grouping in the 9th column.
          --upgrade             Upgrade existing database to current schema
          --gff3_munge          Activate GFF3 name munging (see Bio::DB::GFF)
          --quiet               No progress reports
          --summary             Generate summary statistics for drawing coverage histograms.
                                  This can be run on a previously loaded database or during
                                  the load.

SEE ALSO

       Bio::DB::GFF, bulk_load_gff.pl, bp_load_gff.pl

AUTHOR

       Lincoln Stein, lstein@cshl.org

       Copyright (c) 2002 Cold Spring Harbor Laboratory

       This library is free software; you can redistribute it and/or modify it under the same
       terms as Perl itself.  See DISCLAIMER.txt for disclaimers of warranty.