NAME

       wigeon - reimplementation of the Pintail 16S DNA anomaly detection utility

DESCRIPTION

       WigeoN  examines  the  sequence  conservation  between  a  query  and  a trusted reference
       sequence, both in NAST alignment format. Based on the sequence identity between the  query
       and  the reference sequence, there is an expected amount of variation among the alignment.
       If the observed variation is  greater  than  the  95%  quantile  of  the  distribution  of
       variation observed between non-anomalous sequences, then it is flagged as an anomaly.

       WigeoN  is  a  flexible  command-line based reimplementation of the Pintail algorithm Appl
       Environ Microbiol. 2005 Dec;7112:7724-36.

       WigeoN is useful for flagging chimeras and anomalies only in  near  full-length  16S  rRNA
       sequences. WigeoN lacks sensitivity with sequences less than 1000 bp.

       To run WigeoN, you need NAST-formatted sequences generated by the nast-ier utility.

       WigeoN is  part of the microbiomeutil suite.

OPTIONS

   Required:
       --query_NAST
              multi-fasta file containing query sequences in alignment format

   Optional:
       --db_NAST
              db in NAST format

       --db_FASTA
              db in fasta format (megablast formatted)

       --num_top_hits
              default 1: uses only the single best match.

       --plot

       --DEBUG

       --exec_dir
              cd to exec_dir before running

SEE ALSO

       http://microbiomeutil.sourceforge.net/#A_WigeoN

AUTHOR

       This  manual  page  was written by Andreas Tille <tille@debian.org> but can be freely used
       for any other distribution.