NAME

       Bio::Restriction::Enzyme::MultiCut - A single restriction endonuclease

SYNOPSIS

         # set up a single restriction enzyme. This contains lots of
         # information about the enzyme that is generally parsed from a
         # rebase file and can then be read back

         use Bio::Restriction::Enzyme;

DESCRIPTION

       This module defines a restriction endonuclease class where one object represents one of
       the distinct recognition sites for that enzyme. The method others stores references to
       other objects with alternative sites.

       In this schema each object within an EnzymeCollection can be checked for matching a
       sequence.

       REBASE report notation "Bsp24I (8/13)GACNNNNNNTGG(12/7)" means:

          Bsp24I
                    5'      ^NNNNNNNNGACNNNNNNTGGNNNNNNNNNNNN^   3'
                    3' ^NNNNNNNNNNNNNCTGNNNNNNACCNNNNNNN^        5'

FEEDBACK

   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send
       your comments and suggestions preferably to one of the Bioperl mailing lists. Your
       participation is much appreciated.

         bioperl-l@bioperl.org                  - General discussion
         http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and reponsive experts will
       be able look at the problem and quickly address it. Please include a thorough description
       of the problem with code and data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their
       resolution. Bug reports can be submitted via the web:

         https://redmine.open-bio.org/projects/bioperl/

AUTHOR

       Heikki Lehvaslaiho, heikki-at-bioperl-dot-org

CONTRIBUTORS

       Rob Edwards, redwards@utmem.edu

COPYRIGHT

       Copyright (c) 2003 Rob Edwards.

       Some of this work is Copyright (c) 1997-2002 Steve A. Chervitz. All Rights Reserved.  This
       module is free software; you can redistribute it and/or modify it under the same terms as
       Perl itself.

SEE ALSO

       Bio::Restriction::Enzyme, Bio::Restriction::Analysis, Bio::Restriction::EnzymeCollection

APPENDIX

       Methods beginning with a leading underscore are considered private and are intended for
       internal use by this module. They are not considered part of the public interface and are
       described here for documentation purposes only.

   new
        Title     : new
        Function
        Function  : Initializes the enzyme object
        Returns   : The Restriction::Enzyme::MultiCut object
        Argument  :

   others
        Title     : others
        Usage     : $re->others(@enz_obj_array);
        Function  : Stores auxiliary Enzyme::MultiCut objects for multicutting enzymes
        Arguments : optional array of Enzyme::MultiCut objects
        Returns   : array of Enzyme objects

       Added for compatibility to REBASE

   purge_others
        Title     : purge_others
        Usage     : $re->purge_references();
        Function  : Purges the set of references for this enzyme
        Arguments :
        Returns   :