Provided by: libbio-perl-perl_1.6.923-1_all
NAME
Bio::Search::Result::BlastResult - Blast-specific subclass of Bio::Search::Result::GenericResult
SYNOPSIS
# Working with iterations (PSI-BLAST results) $result->next_iteration(); $result->num_iterations(); $result->iteration(); $result->iterations(); # See Bio::Search::Result::GenericResult for information about working with Results. # See Bio::Search::Iteration::IterationI # for details about working with iterations. # TODO: # * Show how to configure a SearchIO stream so that it generates # BlastResult objects.
DESCRIPTION
This object is a subclass of Bio::Search::Result::GenericResult and provides some operations that facilitate working with BLAST and PSI-BLAST results. For general information about working with Results, see Bio::Search::Result::GenericResult.
FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to the Bioperl mailing list. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track of the bugs and their resolution. Bug reports can be submitted via the web: https://redmine.open-bio.org/projects/bioperl/
AUTHOR - Steve Chervitz
Email sac@bioperl.org
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ new Title : new Usage : my $obj = Bio::Search::Result::BlastResult->new(); Function: Builds a new Bio::Search::Result::BlastResult object Returns : Bio::Search::Result::BlastResult Args : See Bio::Search::Result::GenericResult(); The following parameters are specific to BlastResult: -iterations => array ref of Bio::Search::Iteration::IterationI objects -inclusion_threshold => e-value threshold for inclusion in the PSI-BLAST score matrix model (blastpgp) hits Title : hits Usage : my @hits = $result->hits Function: Returns the available hits for this Result Returns : Array of L<Bio::Search::Hit::HitI> objects Args : none Note : This method overrides L<Bio::Search::Result::GenericResult::hits> to take into account the possibility of multiple iterations, as occurs in PSI-BLAST reports. If there are multiple iterations, all 'new' hits for all iterations are returned. These are the hits that did not occur in a previous iteration. See Also: L<Bio::Search::Result::GenericResult::hits> next_hit Title : next_hit Usage : while( $hit = $result->next_hit()) { ... } Function: Returns the next available Hit object, representing potential matches between the query and various entities from the database. Returns : a Bio::Search::Hit::HitI object or undef if there are no more. Args : none Note : This method overrides L<Bio::Search::Result::GenericResult::next_hit> to take into account the possibility of multiple iterations, as occurs in PSI-BLAST reports. If there are multiple iterations, calling next_hit() traverses the all of the hits, old and new, for each iteration, calling next_hit() on each iteration. See Also: L<Bio::Search::Iteration::GenericIteration::next_hit> num_hits Title : num_hits Usage : my $hitcount= $result->num_hits Function: returns the number of hits for this query result Returns : integer Args : none Note : This method overrides L<Bio::Search::Result::GenericResult::num_hits> to take into account the possibility of multiple iterations, as occurs in PSI-BLAST reports. If there are multiple iterations, calling num_hits() returns the number of 'new' hits for each iteration. These are the hits that did not occur in a previous iteration. See Also: L<Bio::Search::Result::GenericResult::num_hits> add_hit Title : add_hit Usage : $report->add_hit($hit) Function: Adds a HitI to the stored list of hits Returns : Number of HitI currently stored Args : Bio::Search::Hit::HitI add_iteration Title : add_iteration Usage : $report->add_iteration($iteration) Function: Adds a IterationI to the stored list of iterations Returns : Number of IterationI currently stored Args : Bio::Search::Iteration::IterationI next_iteration Title : next_iteration Usage : while( $it = $result->next_iteration()) { ... } Function: Returns the next Iteration object, representing all hits found within a given PSI-Blast iteration. Returns : a Bio::Search::Iteration::IterationI object or undef if there are no more. Args : none iteration Usage : $iteration = $blast->iteration( $number ); Purpose : Get an IterationI object for the specified iteration in the search result (PSI-BLAST). Returns : Bio::Search::Iteration::IterationI object Throws : Bio::Root::NoSuchThing exception if $number is not within range of the number of iterations in this report. Argument : integer (optional, if not specified get the last iteration) First iteration = 1 num_iterations Usage : $num_iterations = $blast->num_iterations; Purpose : Get the number of iterations in the search result (PSI-BLAST). Returns : Total number of iterations in the report Argument : none (read-only) number_of_iterations Usage : $num_iterations = $blast->number_of_iterations; Purpose : Get the number of iterations in the search result (PSI-BLAST). Returns : Total number of iterations in the report Argument : none (read-only) Note : Alias of L<num_iterations>. round Usage : $round = $blast->round( $number ); Purpose : Get an IterationI object for the specified iteration in the search result (PSI-BLAST). Returns : Bio::Search::Iteration::IterationI object Throws : Bio::Root::NoSuchThing exception if $number is not within range of the number of iterations in this report. Argument : integer (optional, if not specified get the last iteration) First iteration = 1 Note : Alias of L<iteration>. iterations Title : iterations Usage : my @iterations = $result->iterations Function: Returns the IterationI objects contained within this Result Returns : Array of L<Bio::Search::Iteration::IterationI> objects Args : none psiblast Usage : if( $blast->psiblast ) { ... } Purpose : Set/get a boolean indicator whether or not the report is a PSI-BLAST report. Returns : 1 if PSI-BLAST, undef if not. Argument : 1 (when setting) no_hits_found Usage : $nohits = $blast->no_hits_found( $iteration_number ); Purpose : Get boolean indicator indicating whether or not any hits were present in the report. This is NOT the same as determining the number of hits via the hits() method, which will return zero hits if there were no hits in the report or if all hits were filtered out during the parse. Thus, this method can be used to distinguish these possibilities for hitless reports generated when filtering. Returns : Boolean Argument : (optional) integer indicating the iteration number (PSI-BLAST) If iteration number is not specified and this is a PSI-BLAST result, then this method will return true only if all iterations had no hits found. set_no_hits_found Usage : $blast->set_no_hits_found( $iteration_number ); Purpose : Set boolean indicator indicating whether or not any hits were present in the report. Returns : n/a Argument : (optional) integer indicating the iteration number (PSI-BLAST) _next_iteration_index Title : _next_iteration_index Usage : private rewind Title : rewind Usage : $result->rewind; Function: Allow one to reset the Iteration iterator to the beginning Since this is an in-memory implementation Returns : none Args : none inclusion_threshold Title : inclusion_threshold Usage : my $incl_thresh = $result->inclusion_threshold; (read-only) Function: Gets the e-value threshold for inclusion in the PSI-BLAST score matrix model (blastpgp) that was used for generating the report being parsed. Returns : number (real) or undef if not a PSI-BLAST report. Args : none