Provided by: libbio-perl-run-perl_1.6.9-2_all
NAME
Bio::Tools::Run::Alignment::Exonerate
SYNOPSIS
use Bio::Tools::Run::Alignment::Exonerate; use Bio::SeqIO; my $qio = Bio::SeqIO->new(-file=>$ARGV[0],-format=>'fasta'); my $query = $qio->next_seq(); my $tio = Bio::SeqIO->new(-file=>$ARGV[1],-format=>'fasta'); my $target = $sio->next_seq(); #exonerate parameters can all be passed via arguments parameter. #parameters passed are not checked for validity my $run = Bio::Tools::Run::Alignment::Exonerate-> new(arguments=>'--model est2genome --bestn 10'); my $searchio_obj = $run->run($query,$target); while(my $result = $searchio->next_result){ while( my $hit = $result->next_hit ) { while( my $hsp = $hit->next_hsp ) { print $hsp->start."\t".$hsp->end."\n"; } } }
DESCRIPTION
Wrapper for Exonerate alignment program. You can get exonerate at http://www.ebi.ac.uk/~guy/exonerate/. This wrapper is written without parameter checking. All parameters are passed via the arugment parameter that is passed in the constructor. See SYNOPSIS. For exonerate parameters, run exonerate --help for more details.
PROGRAM VERSIONS
The tests have been shown to pass with exonorate versions 2.0 - 2.2.
FEEDBACK
Mailing Lists User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments and suggestions preferably to one of the Bioperl mailing lists. Your participation is much appreciated. bioperl-l@bioperl.org - General discussion http://bioperl.org/wiki/Mailing_lists - About the mailing lists Support Please direct usage questions or support issues to the mailing list: bioperl-l@bioperl.org rather than to the module maintainer directly. Many experienced and reponsive experts will be able look at the problem and quickly address it. Please include a thorough description of the problem with code and data examples if at all possible. Reporting Bugs Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution. Bug reports can be submitted via the web: http://redmine.open-bio.org/projects/bioperl/
AUTHOR - Shawn Hoon
Email shawnh-at-stanford.edu
APPENDIX
The rest of the documentation details each of the object methods. Internal methods are usually preceded with a _ program_name Title : program_name Usage : $factory>program_name() Function: holds the program name Returns: string Args : None program_dir Title : program_dir Usage : $factory->program_dir(@params) Function: returns the program directory, obtained from ENV variable. Returns: string Args : new Title : new Usage : my $factory= Bio::Tools::Run::Phrap->new(); Function: creates a new Phrap factory Returns: Bio::Tools::Run::Phrap Args : version Title : version Usage : exit if $prog->version() < 1.8 Function: Determine the version number of the program Example : Returns : float or undef Args : none run Title : run() Usage : my $feats = $factory->run($seq) Function: Runs Phrap Returns : An array of Bio::SeqFeature::Generic objects Args : A Bio::PrimarySeqI _input Title : _input Usage : $factory->_input($seqFile) Function: get/set for input file Returns : Args : _run Title : _run Usage : $factory->_run() Function: Makes a system call and runs Phrap Returns : An array of Bio::SeqFeature::Generic objects Args : _writeInput Title : _writeInput Usage : $factory->_writeInput($query,$target) Function: Creates a file from the given seq object Returns : A string(filename) Args : Bio::PrimarySeqI _setparams Title : _setparams Usage : Internal function, not to be called directly Function: creates a string of params to be used in the command string Example : Returns : string of params Args :