NAME

       glam2-purge - Removes redundant sequences from a FASTA file

SYNOPSIS

       glam2-purge file score [options]

DESCRIPTION

       glam2-purge is a modified version of Andrew Neuwald´s purge program that removes redundant
       sequences from a FASTA file. This is recommended in order to prevent highly similar
       sequences distorting the search for motifs. Purge works with either DNA or protein
       sequences and creates an output file such that no two sequences have a (gapless) local
       alignment score greater than a threshold specified by the user. The output file is named
       <file>.<score>. The alignment score is based on the BLOSUM62 matrix for proteins, and on a
       +5/-1 scoring scheme for DNA. Purge can also be used to mask tandem repeats. It uses the
       XNU program for this purpose.

OPTIONS

       -n
           Sequences are DNA (default: protein).

       -b
           Use blast heuristic method (default for protein).

       -e
           Use an exhaustive method (default for DNA).

       -q
           Keep first sequence in the set.

       -x
           Use xnu to mask protein tandem repeats.

SEE ALSO

       glam2(1), glam2format(1), glam2mask(1), glam2scan(1), xnu(1)

       The full Hypertext documentation of GLAM2 is available online at
       http://bioinformatics.org.au/glam2/ or on this computer in /usr/share/doc/glam2/.

REFERENCES

       Purge was written by Andy Neuwald and is described in more detail in Neuwald et al.,
       "Gibbs motif sampling: detection of bacterial outer membrane protein repeats", Protein
       Science, 4:1618–1632, 1995. Please cite it if you use Purge.

       If you use GLAM2, please cite: MC Frith, NFW Saunders, B Kobe, TL Bailey (2008)
       Discovering sequence motifs with arbitrary insertions and deletions, PLoS Computational
       Biology (in press).

AUTHORS

       Andrew Neuwald
           Author of purge, renamed glam2-purge in Debian.

       Martin Frith
           Modified purge to be ANSI standard C and improved the user interface.

       Timothy Bailey
           Modified purge to be ANSI standard C and improved the user interface.

       Charles Plessy <plessy@debian.org>
           Formatted this manpage in DocBook XML for the Debian distribution.

COPYRIGHT

       The source code and the documentation of Purge and GLAM2 are released in the public
       domain.