NAME

       prima - Selects primers for PCR and DNA amplification.

SYNOPSIS

       prima -sequence sequence -targetrange toggle -targetstart integer -targetend integer -mintmprimer float
             -maxtmprimer float -minplen integer -maxplen integer -minpgccont float -maxpgccont float
             [-primer toggle] -minprimerlen integer -maxprimerlen integer -minpmgccont float -maxpmgccont float
             -overlap integer -saltconc float -dnaconc float -list boolean -outfile outfile

       prima -help

DESCRIPTION

       prima is a command line program from EMBOSS (“the European Molecular Biology Open Software Suite”). It is
       part of the "Nucleic:Primers" command group(s).

OPTIONS

   Input section
       -sequence sequence

   Required section
   Target options
       -targetrange toggle
           Default value: N

       -targetstart integer
           Default value: $(sequence.begin)

       -targetend integer
           Default value: $(sequence.length)

       -mintmprimer float
           Default value: 53

       -maxtmprimer float
           Default value: 58

   Additional section
   Product options
       -minplen integer
           Default value: 100

       -maxplen integer
           Default value: 300

       -minpgccont float
           Default value: .40

       -maxpgccont float
           Default value: .55

   Primer options
       -primer toggle
           Specify primer properties Default value: Y

       -minprimerlen integer
           Default value: 18

       -maxprimerlen integer
           Default value: 22

       -minpmgccont float
           Default value: .40

       -maxpmgccont float
           Default value: .55

   Advanced section
       -overlap integer
           Default value: 50

       -saltconc float
           Default value: 50

       -dnaconc float
           Default value: 50

       -list boolean
           Default value: N

   Output section
       -outfile outfile

BUGS

       Bugs can be reported to the Debian Bug Tracking system (http://bugs.debian.org/emboss), or directly to
       the EMBOSS developers (http://sourceforge.net/tracker/?group_id=93650&atid=605031).

SEE ALSO

       prima is fully documented via the tfm(1) system.

AUTHOR

       Debian Med Packaging Team <debian-med-packaging@lists.alioth.debian.org>
           Wrote the script used to autogenerate this manual page.

COPYRIGHT

       This manual page was autogenerated from an Ajax Control Definition of the EMBOSS package. It can be
       redistributed under the same terms as EMBOSS itself.

EMBOSS 6.4.0                                       05/11/2012                                          PRIMA(1e)