Provided by: subread_1.5.0-p1+dfsg-2_amd64
NAME
qualityScores - a component of subread suite
USAGE
qualityScores [options] -i <input_file> -o <output_file> Required arguments: -i <string> Name of input file including read data. The default format is Fastq. -o <string> Name of output file that is a text file including Phred scores for each read base. Optional arguments: --gzFASTQinput Input file is in gzipped Fastq format. --BAMinput Input file is in BAM format. --SAMinput Input file is in SAM format. --first-end Use only first reads in paired-end data. Only applicable for paired-end BAM/SAM input. --second-end Use only second reads in paired-end data. Only applicable for paired-end BAM/SAM input. --counted-reads <int> Total number of reads to be extracted from the input file. 10,000 by default. --phred-offset <33|64> refer to subread aligner.