NAME

       Bio::Tools::Analysis::Protein::GOR4 - a wrapper around GOR4 protein secondary structure prediction server

SYNOPSIS

         use Bio::Tools::Analysis::Protein::GOR4;
         #get a Bio::Seq or Bio::PrimarySeq
         use Bio::PrimarySeq;
         $seq = Bio::PrimarySeq->new
           (-seq=>'IKLCVHHJHJHJHJHJHJHNLAILAKAHLIELALAL',
            -primary_id=>'test'); # a Bio::PrimarySeqI object

         my $gor4 = Bio::Tools::Analysis::Protein::GOR4->new (-seq=>$seq);
         $gor4->run;
         print $gor4->result;# #raw text to standard error

DESCRIPTION

       A module to remotely retrieve predictions of protein secondary structure.  Each residue in the protein
       receives a score representing the likelihood of existing in each of three different states (helix, coil
       or sheet), e.g.,

         my $analysis_object = Bio::Tools::SimpleAnalysis::Protein::GOR4->
             new(-seq => $seq);

       creates a new object

         $analysis_object->run;

       submits the query to the server and obtains raw text output

       Given an amino acid sequence the results can be obtained in 4 formats, determined by the argument to the
       result method

       1.  The raw text of the program output

             my $rawdata = $analysis_object->result;

       2.  An reference to an array of hashes of scores for each state and the assigned state.

             my $data_ref = $analysis_object->result('parsed');
             print "score for helix at residue 2 is $data_ref->[1]{'helix'}\n";
             print "predicted struc  at residue 2 is $data_ref->[1]{'struc}\n";

       3.  An array of Bio::SeqFeature::Generic objects where each feature is a predicted unit of secondary
           structure. Only stretches of helix/sheet predictions for longer than 4 residues are defined as
           helices. See Bio::Tools::Analysis::Domcut.pm for examples of how to add sequence features.

             my @fts = $analysis_object->result(Bio::SeqFeatureI);
             for my $ft (@fts) {
                 print " From ",  $ft->start, " to  ",$ft->end, " struc: " ,
                        ($ft->each_tag_value('type'))[0]  ,"\n";
             }

       4.  A Bio::Seq::Meta::Array implementing sequence.

           This is a Bio::Seq object that can also hold data about each residue in the sequence In this case,
           the sequence can be associated with a single array of GOR4 prediction scores.  e.g.,

             my $meta_sequence = $analysis_object->result('all');
             print "helix scores from residues 10-20 are ",
                 $meta_sequence->named_submeta_text("GOR4_helix",10,20), "\n";

           Meta sequence names are : GOR4_helix, GOR4_sheet, GOR4_coil, GOR4_struc, representing the scores for
           each residue.

           Many methods common to all analyses are inherited from Bio::Tools::Analysis::SimpleAnalysisBase.

SEE ALSO

       Bio::SimpleAnalysisI, Bio::Tools::Analysis::SimpleAnalysisBase, Bio::Seq::Meta::Array, Bio::WebAgent

FEEDBACK

   Mailing Lists
       User feedback is an integral part of the evolution of this and other Bioperl modules. Send your comments
       and suggestions preferably to one of the Bioperl mailing lists.  Your participation is much appreciated.

         bioperl-l@bioperl.org                  - General discussion
         http://bioperl.org/wiki/Mailing_lists  - About the mailing lists

   Support
       Please direct usage questions or support issues to the mailing list:

       bioperl-l@bioperl.org

       rather than to the module maintainer directly. Many experienced and reponsive experts will be able look
       at the problem and quickly address it. Please include a thorough description of the problem with code and
       data examples if at all possible.

   Reporting Bugs
       Report bugs to the Bioperl bug tracking system to help us keep track the bugs and their resolution.  Bug
       reports can be submitted via the web:

         https://github.com/bioperl/bioperl-live/issues

AUTHORS

       Richard Adams, Richard.Adams@ed.ac.uk,

APPENDIX

       The rest of the documentation details each of the object methods. Internal methods are usually preceded
       with a _

   result
        Name    : result
        Usage   : $job->result (...)
        Returns : a result created by running an analysis
        Args    : see keys of $RESULT_SPEC

       The method returns a result of an executed job. If the job was terminated by an error the result may
       contain an error message instead of the real data.

       This implementation returns differently processed data depending on argument:

       undef
          Returns the raw ASCII data stream but without HTML tags

       'Bio::SeqFeatureI'
          The argument string defines the type of bioperl objects returned in an array.  The objects are
          Bio::SeqFeature::Generic.  Feature primary tag is "2ary".  Feature tags are "type" (which can be
          helix, sheet or coil) "method" (GOR4).

       'parsed'
          Array of hash references of { helix =>, sheet => , coil => , struc=>}.

       'meta'
          A Bio::Seq::Meta::Array object. Scores can be accessed using methods from this class. Meta sequence
          names are GOR4_helix, GOR4_sheet, GOR4_coil, GOR4_struc.